TACC3基因在肝癌中的表达及其与患者的预后关系

Relationship of TACC3 gene expression with prognosis in hepatocellular carcinoma

背景转录相关酸性卷曲蛋白3(transforming acidic coiled coil protein 3,TACC3)是TACC家族的重要成员,研究表明,TACC3基因在乳腺癌,非小细胞肺癌,胃癌中高表达,并与预后较差有关.但是其在肝癌中的表达情况及与患者预后的关系鲜见报道.目的通过生物信息学手段探讨TACC3基因在肝癌中的表达及其与患者的预后关系.方法分别检索BioGPS、Oncomine、癌症细胞系百科全书(cancer cell line encyclopedia,CCLE)数据库,挖掘分析TACC3基因在正常人体组织、肝癌组织及肝癌细胞系中的表达情况,应用Kaplan-Meier Plotter、GEPIA等数据库分析TACC3基因对肝癌患者预后的影响.结果BioGPS数据库分析结果显示TACC3基因在人体各组织中均有表达,肝脏组织中的表达值中位数略高于其他正常组织(8.95 vs 7.1).从Oncomine数据库检索出TACC3基因相关研究290项,显示肝癌组织中TACC3基因高表达4项,低表达1项,Meta分析显示与正常肝组织相比TACC3基因在肝癌组织中呈高表达状态[中位秩(Median Rank)=442.5,P<0.05].CCLE数据库分析显示,在肝癌细胞系中TACC3 mRNA高表达.Kaplan-Meier Plotter及GEPIA数据库生存分析结果显示,TACC3基因高表达组的肝癌患者总体生存时间(OS)和无进展生存时间(PFS)等生存预后指标均较低表达组差(P<0.05).结论TACC3基因在肝癌组织中呈高表达,且TACC3基因高表达与肝癌患者生存预后差有关.

BACKGROUND Transforming acidic coiled coil protein 3(TACC3)is an important member of the TACC family.Studies have shown that TACC3 gene is highly expressed in breast cancer,non-small cell lung cancer,and gastric cancer,and is associated with poor prognosis.However,its expression in liver cancer and its relationship with prognosis are rarely reported.AIM To explore the clinical significance of TACC3 gene expression in liver cancer.METHODS The expression of TACC3 gene in normal human tissues,liver cancer tissues,and liver cancer cell lines was mined by searching databases including BioGPS,Oncomine,and Cancer Cell Line Encyclopedia(CCLE),respectively.Kaplan-Meier plotter and GEPIA were used to analyze the effect of TACC3 gene expression on the prognosis of liver cancer patients.RESULTS BioGPS database analysis showed that TACC3 gene was expressed in all normal tissues and TACC3 gene expression in the liver was slightly higher than that in other normal tissues(median expression value,8.95 vs 7.1).A total of 290 studies on TACC3 gene were retrieved from Oncomine database,showing four studies with high expression and one with low expression of TACC3 gene in liver cancer tissues.Meta-analysis showed that TACC3 gene was highly expressed in liver cancer tissues compared with normal liver tissues(Median rank=442.5,P<0.05).CCLE database analysis showed that TACC3 mRNA was highly expressed in liver cancer cell lines.The survival analysis results by Kaplan-Meier plotter based on the GEPIA database showed that the overall survival time(OS)and progression-free survival time(PFS)of liver cancer patients in the TACC3 high expression group were worse than those of the low expression group(P<0.05).CONCLUSION TACC3 gene is highly expressed in liver cancer tissues.And the high expression of TACC3 gene is associated with poor survival prognosis in liver cancer patients.