哈萨克族食管鳞癌中hMSH2及FHIT表达的临床病理学意义

Clinicopathological significance of hMSH2 and FHIT expression in Kazakh esophageal squamous cell carcinoma

目的研究哈萨克族食管鳞癌中hMSH2与FHIT基因的表达,分析基因表达的相关性及其临床病理意义。方法选取2013年5月—2017年5月新疆医科大学第一附属医院行食管癌根治术43例患者的食管鳞癌组织及其癌旁正常组织标本,30例采用qRT-PCR方法,43例进行免疫组织化学法检测hMSH2和FHIT基因的表达水平与临床病理特征的关系。结果食管鳞癌组织中hMSH2和FHIT两个基因的mRNA的表达水平显著低于癌旁正常组织,差异有统计学意义(P<0.05)。免疫组织化学结果显示hMSH2、FHIT蛋白在食管鳞癌组织中阳性表达率显著低于癌旁正常组织,差异有统计学意义(P<0.05)。hMSH2蛋白表达水平与肿瘤分化程度、淋巴结转移及术后病理分期有关(P<0.05),与食管癌患者的年龄、性别及肿瘤部位无关(P>0.05),而FHIT表达水平仅与浸润深度有关(P<0.05)。Kaplan-Meier生存分析发现,食管癌hMSH2表达水平与患者生存率相关(P<0.05)。COX多因素生存分析结果显示,hMSH2基因表达缺失及肿瘤分化程度是哈萨克族食管鳞癌患者不良预后的独立危险因素(P<0.05)。结论hMSH2和FHIT可能参与了哈萨克族食管鳞癌的发生、发展,联合表达的检测可作为预测预后的重要指标。

Objective To investigate the expression of hMSH2 and FHIT genes in Kazakh esophageal squamous cell carcinoma,and analyze the correlation between gene expression and its clinicopathological significance.Methods Esophageal cancer and its adjacent normal tissue specimens were selected from 43 patients who underwent radical resection of esophageal cancer in the First Affiliated Hospital of Xinjiang Medical University from May 2013 to May 2017.qRT-PCR method was used on 30 cases,while immunohistochemistry was conducted on 43 cases to study their clinicopathological characteristics.Results The mRNA expression levels of both hMSH2 and FHIT genes in esophageal squamous cell carcinoma tissues were significantly lower than those in adjacent normal tissues,and the differences were statistically significant(P<0.05).Results of immunohistochemistry showed that the positive expression rate of hMSH2 and FHIT protein in esophageal squamous cell carcinoma tissues was significantly lower than that in adjacent normal tissues,and the difference was statistically significant(P<0.05).The expression level of HMSH2 protein was related to the degree of tumor differentiation,lymph node metastasis and postoperative pathological stage(P<0.05),while it had nothing to do with the age,sex and tumor location of the patients with esophageal cancer(P>0.05).The expression level of FHIT was only related to the depth of infiltration(P<0.05).Kaplan-Meier survival analysis found that the expression level of HMSH2 in esophageal cancer was correlated with survival rate(P<0.05).The results of COX multivariate survival analysis showed that the loss of hMSH2 gene expression and the degree of tumor differentiation were independent risk factors for poor prognosis in Kazakh patients with esophageal squamous cell carcinoma(P<0.05).Conclusion hMSH2 and FHIT may be involved in the occurrence and development of Kazakh esophageal cancer,so the detection of co-expression could be used as an important indicator for prognosis.