Neuropilin-1表达对口腔鳞状细胞癌放疗敏感性的影响

Effect of Neuropilin-1 Expression on Radiotherapy-sensitivity of Oral Squamous Cell Carcinoma

目的:探究Neuropilin-1表达对口腔鳞状细胞癌放疗敏感性的影响。方法:挖掘基因表达汇编(Gene Expression Omnibus,GEO)数据库,筛选出口腔鳞状细胞癌放疗耐受的差异基因,再利用癌症基因图谱(The Cancer Genome Atlas,TCGA)数据库探讨关键基因在口腔鳞状细胞癌中的表达及对生存率的影响。通过构建敲低、过表达细胞系,探讨该基因对口腔鳞状细胞癌Tca8113细胞增殖及放疗敏感性的影响。结果:GEO数据库筛选出Neuropilin-1为口腔鳞状细胞癌的上调基因,TCGA数据库结果分析,Neuropilin-1在口腔鳞状细胞癌中表达量较正常口腔组织高(P<0.01),差异有统计学意义。且在口腔鳞状细胞癌中Neuropilin-1的表达量与肿瘤恶性程度正相关。细胞功能学研究提示,Neuropilin-1表达能促进Tca8113细胞的增殖和降低Tca8113细胞对射线的敏感性。结论:Neuropilin-1与口腔鳞状细胞癌的发生发展,放疗耐受相关,是逆转口腔鳞状细胞癌放疗不敏感的潜在靶点。

Objective:To investigate the effect of Neuropilin-1 expression on radiotherapy-sensitivity of oral squamous cell carcinoma.Methods:The GEO database method was used to screen out the differential gene for radiotherapy resistance in oral squamous cell carcinoma.The TCGA database was used to explore the expression of key genes in oral squamous cell carcinoma and its effect on survival rate.By constructing knockdown and overexpression cell lines,the effects of this gene on cell proliferation and radiation sensitivity Tca8113 oral squamous cell carcinoma were investigated.Results:Neuropilin-1 was selected as an up-regulated gene in oral squamous cell carcinoma by GEO database.The expression of Neuropilin-1 in oral squamous cell carcinoma was higher than that in normal oral tissues(P<0.01).Moreover,the expression level of Neuropilin-1 in oral squamous cell carcinoma is positively correlated with the degree of tumor malignancy.Cellular functional studies suggest that Neuropilin-1 expression promotes the proliferation of Tca8113 cells and reduces the sensitivity of Tca8113 cells to radiation.Conclusion:Neuropilin-1 is associated with the development of oral squamous cell carcinoma and is associated with radiotherapy tolerance.It is a potential target for reversing the insensitivity of oral squamous cell carcinoma to radiotherapy.