基于可溶性胶原的牙周缓释剂体外抑菌对照实验研究

Antibacterial activity of periodontal sustained-release agent based on atelocollagen in vitro

目的:研究可溶性胶原作为缓释材料的牙周缓释剂对4种牙周致病菌的抑菌作用,为研制安全有效的牙周缓释剂提供实验基础。方法:制备可溶性胶原二甲胺四环素、环丙沙星缓释剂,并将药物等比稀释至10个浓度。测量缓释剂对牙龈卟啉单胞菌、中间型普氏菌、具核梭杆菌、粘性放线菌的最低抑菌浓度(MIC),用钢管法测量缓释剂的抑菌环直径。结果:环丙沙星组对4种菌株的最低抑菌浓度小于0.009mg/mL,二甲胺四环素组对4种菌株的最低抑菌浓度小于0.003mg/mL,且在药物浓度较低时(<0.009mg/mL),胶原浓度相同(2%)的环丙沙星组和二甲胺四环素组相比,二甲胺四环素组对4种牙周致病菌的抑菌能力普遍要高于环丙沙星组。对于牙龈卟啉单胞菌,药物浓度小于0.781mg/mL时,可溶性胶原缓释剂组的抑菌能力高于盐酸米诺环素软膏(派丽奥)(P<0.05)。对于中间型普氏菌,当药物浓度大于2.344 mg/mL时,胶原浓度为3.5%的二甲胺四环素组抑菌作用最强,且在药物浓度逐渐降低后(<0.086mg/mL),盐酸米诺环素组的抑菌能力低于可溶性胶原二甲胺四环素组(P<0.05)。对于具核梭杆菌和粘性放线菌,也出现了在药物浓度较低时(<0.009mg/mL),可溶性胶原二甲胺四环素组的抑菌作用要高于盐酸米诺环素组(P<0.05)的现象。结论:可溶性胶原二甲胺四环素组具有比可溶性胶原环丙沙星组更强的抑菌作用。可溶性胶原缓释剂具有良好的抑菌作用,且在药物浓度降低时,可溶性胶原良好的缓释作用保证了牙周缓释剂稳定的抑菌效能。

Objective:To investigate the bacteriostatic effect of atelocollagen sustained-release agent on four periodontal pathogens and to provide experimental basis for the development of a new type of safe periodontal sustained-release agent.Methods:Prepare atelocollagen-minocycline and atelocollagen-ciprofloxacin sustained-release agents.The minimal inhibitory concentration(MIC)and inhibitory zone against four standard strains:Porphyomonasgingivalis(P.g),Prevotella intermedia(P.i),Fusobacterium nucleatum(F.n)and Actinomyces viscosus(A.v)were measured by proportional dilution and Oxford cup method.Result:The MIC of ciprofloxacin group was less than 0.009 mg/mL,and the MIC of minocycline group was less than 0.003 mg/mL.Compared with minocycline group,the antibacterial activity of ciprofloxacin groupwas weaker when the drug concentration was lower than 0.009 mg/mL.For P.g,when the drug concentration was less than 0.781mg/ml,the antibacterial ability of the atelocollagen sustained-release agent was higher than that of Periocline(P<0.05).For P.i,atelocollagen of 3.5%concentration had the strongest antibacterial effect when drug concentration was more than 2.344mg/ml.After the drug concentration gradually decreased,the antibacterial ability of Periocline group was lower than that of atelocollagen-minocycline group(P<0.05).For F.n and A.v,the antibacterial effect of atelocollagen-minocycline group was higher than that of Periocline group(P<0.05)when the drug concentration was lower than 0.009mg/ml.Conclusion:Atelocollagen-minocycline group has stronger antibacterial effect than atelocollagen-ciprofloxacin group.Atelocollagen sustained-release agent has marked antibacterial effect,and the good sustained-release effect of atelocollagen ensures the stable antibacterial effect of periodontal sustained-release agent when the drug concentration is reduced.