寨卡病毒E蛋白亚单位疫苗复合型佐剂的筛选

Screening of compound adjuvants for subunit vaccine of ZIKV E protein

为了筛选能够增强亚单位疫苗免疫效果的复合型免疫佐剂,试验以前期筛选的ZJ01和ZJ02佐剂为基础,分别与免疫增强剂A、B、C、D混匀制备复合型免疫佐剂,与表达寨卡病毒(ZIKV)E蛋白的亚单位疫苗混匀后免疫Balb/c小鼠,检测免疫后小鼠IgG、IgG1、IgG2a、IgG2b抗体水平,ZIKV E蛋白特异性抗体和中和抗体水平及淋巴细胞增殖效果,以验证复合型佐剂的免疫增强效果。结果表明:免疫后第35天,ZJ01和ZJ02试验组含有A组分和D组分的血清中IgG、IgG1、IgG2a、IgG2b、特异性抗体及中和抗体等抗体水平多数显著或极显著高于E蛋白对照组(P<0.05或P<0.01、P<0.001、P<0.0001),其中IgG、IgG1、IgG2a、IgG2b抗体水平是E蛋白对照组的1.74~3.35倍;特异性抗体水平是E蛋白对照组的1.43~1.60倍;中和抗体水平是E蛋白对照组的1.33~1.75倍;T淋巴细胞增殖水平是E蛋白对照组的1.30~1.36倍。说明含有A组分和D组分的ZJ01与ZJ02复合型佐剂能更好地增强亚单位疫苗的免疫效果。

In order to screen for compound immune adjuvants that could enhance the immune effect of subunit vaccines,the ZJ01 and ZJ02 adjuvants screened in the previous study were used and were mixed with immunopotentiators A,B,C,and D respectively to prepare compound adjuvants.The compound adjuvants were then mixed with a subunit vaccine of ZIKV E protein for Balb/c mice immunization to detect the levels of IgG,IgG1,IgG2a,and IgG2b antibodies,the levels of ZIKV E protein specific antibodies and neutralizing antibodies,and the effect of lymphocyte proliferation in mice after immunization,and to verify the immune enhancement effect of the compound adjuvants.The results showed that on the 35 th day after immunization,the serum levels of IgG,IgG1,IgG2a,IgG2b antibodies,specific antibodies and neutralizing antibodies in the ZJ01 and ZJ02 test groups containing components A and D were mostly significantly or extremely significantly higher than E Protein control group(P<0.05 or P<0.01,P<0.001,P<0.0001),in which IgG,IgG1,IgG2a,and IgG2b antibody levels were 1.74-3.35 times than those of the E protein control group;the specific antibody level was 1.43-1.60 times than that of E protein control group;the neutralizing antibody level was 1.33-1.75 times than that of the E protein control group;the T lymphocyte proliferation level was 1.30-1.36 times than that of the E protein control group.The results indicated that the compound adjuvants of ZJ01 and ZJ02 containing A and D components could better enhance the immune effect of subunit vaccine.