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CRISPR/Cas9基因编辑技术在作物中的应用 被引量：7

Application of CRISPR/Cas9 Gene Editing Technology in Crops
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摘要 CRISPR/Cas9是由小导向RNA介导的基因组定向编辑技术。自2005年以来,该技术得到飞速发展,在生物学、医学和作物遗传育种等多个学科得到广泛应用。与以往的大范围核酸酶、锌指核酸酶(ZFNs)及类转录激活因子效应物核酸酶(TALENs)相比,CRISPR/Cas9技术具有独特的优越性,以其简便的操作和极高的编辑效率成为目前最主要的基因编辑技术。本文系统阐述了CRISPR/Cas9技术的起源发展、基因编辑特点,总结了该技术在作物基因编辑和其他方面的应用,旨在为利用该技术进行农作物种质创新、基因挖掘和育种提供参考。 CRISPR/Cas9 is a genome directed editing technique mediated by small guide RNA. Since 2005, the technology has been developed rapidly and was widely used in biology, medicine and crop genetics and breeding. Compared with meganucleases, zinc finger nucleases(ZFNs) and transcription-like activator effector nucleases(TALENs), CRISPR/Cas9 technology has unsurpassed advantages and has become the most important gene editing technology at present due to its simple operation and high editing efficiency. This paper systematically described the origin, development and the characteristics of CRISPR/Cas9 gene editing, summarized the application of this technology in crop gene editing and other aspects. The purpose of this paper is to provide reference for crop germplasms innovation, gene mining and breeding.
作者 林萌萌 李春娟 闫彩霞 孙全喜 赵小波 王娟 苑翠玲 单世华 LIN Mengmeng;LI Chunjuan;YAN Caixia;SUN Quanxi;ZHAO Xiaobo;WANG Juan;YUAN Cuiling;SHAN Shihua(Shandong Peanut Research Institute,Qingdao,Shandong 266100)
机构地区 山东省花生研究所
出处 《核农学报》 CAS CSCD 北大核心 2021年第6期1329-1339,共11页 Journal of Nuclear Agricultural Sciences
基金 泰山学者特聘专家(ts201712080) 山东省现代农业产业技术体系(SDAIT-04-02) 山东省农业科学院农业科技创新工程(CXGC2016A01) 农业部科研杰出人才及其创新团队培养(13190194)。
关键词 基因编辑 基因突变 作物遗传育种 CRISPR/Cas9 gene editing gene mutation crop genetics and breeding CRISPR/Cas9
分类号 S188 [农业科学—农业基础科学]
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参考文献13

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二级参考文献81

共引文献1210

同被引文献138

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二级引证文献39

核农学报
2021年 第6期

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