丹栀逍遥散减轻慢性心理应激模型小鼠的瘙痒症状机制研究

Danzhi Xiaoyao powder reduced the scratching behavior exaggerated by chronic psychological stress in mice

目的探究丹栀逍遥散治疗心理因素恶化皮肤瘙痒的疗效及可能的作用机制。方法将雄性BALB/c小鼠分为空白组、心理压力组、心理压力+丹栀逍遥散组,除空白组外其余两组小鼠暴露于1小时的慢性避水应激(water avoidance stress,WAS)中,连续10天,丹栀逍遥散组每天予丹栀逍遥散中药灌胃,其余两组予等量生理盐水灌胃。在最后一次应激结束24小时之后,将N-甲基-对甲氧基苯乙胺与甲醛的缩合产物(compound 48/80)注射到小鼠颈后,观察它们的搔抓行为120分钟,记录其搔抓次数,检测皮肤肥大细胞数量及血浆中组胺水平,并通过空场试验、强迫游泳试验观察小鼠治疗前后抑郁行为学改变。结果心理压力+丹栀逍遥散组小鼠搔抓次数明显少于心理压力组(P<0.05),但高于空白组(P<0.05);心理压力组肥大细胞数多于空白组(P<0.05),心理压力+丹栀逍遥散组肥大细胞数量与其余两组均无统计学差异(P>0.05),且心理压力+丹栀逍遥散组血浆组胺水平亦低于心理压力组(P<0.05);在抑郁行为学上,心理压力+丹栀逍遥散组小鼠在空场试验中直立次数多于心理压力组少于空白组(P<0.05),强迫游泳试验中不动时间少于心理压力组多于空白组(P<0.05)。结论丹栀逍遥散可缓解WAS影响下的小鼠的瘙痒症状,其可能的机制是降低了组胺的释放及改善小鼠的抑郁状态。

Objective To explore the efficacy and possible mechanism of Danzhi Xiaoyao powder in the treatment of scratching behavior worsened by psychological factors.Methods Male BALB/c mice were divided into control group(CON group),chronic WAS group(WAS group)and chronic WAS+Danzhi Xiaoyao powder group(DZX group).All mice excluded control group were exposed to 1h water avoidance stress(WAS)for ten consecutive days.DZX group were given Danzhi Xiaoyao powder solution by intragastric administration every day,and the other two groups were given the same amount of saline.Twenty-four hours after the last stress session,the mice were injected into the back of the neck with a condensation product of N-methyl-p-methoxyphenethylamine with formaldehyde(compound 48/80),and their scratching behavior was then observed for 120 min.The scratching number was recorded,the histamine levels in the plasma were examined,and the behavioral changes of depression in mice before and after treatment were observed by open field test and forced swimming test.Results The scratching number was significantly higher in the WAS group than in the DZX group,which still higher than in the CON group.The peak histamine in the plasma after the compound 48/80 injection were also significantly higher in the WAS group in comparison to the DZX group.In terms of depression behavior,the number of standing in the DZX group was more than that in the WAS group and less than that in the CON group,and the immobility time in the forced swimming test was less than that in the WAS group and more than that in the CON group.Conclusion Danzhi Xiaoyao powder can relieve the scratching behavior of mice under the influence of WAS,and its possible mechanism is to control the release of histamine and improve the depressive state of mice.