microRNA-27a在裸鼠体内对宫颈癌细胞的增殖作用

miRNA-27a inhibited the proliferation of cervical adenocarcinoma cells in nude mice

目的研究microRNA-27a在宫颈癌裸鼠皮下种植瘤生长中的作用。方法建立裸鼠皮下种植瘤模型,随机平均分为3组,实验组皮下种植瘤内注射microRNA-27a-antagomir,特异性抑制microRNA-27a的表达,阴性对照组皮下瘤内注射microRNA-27a-antagomir-NC、空白对照组皮下瘤内注射PBS,平均每周2次。每3天用游标卡尺测量皮下种植瘤长径和短径,计算瘤体体积,绘制生长曲线。最后一次注射后1周,处死裸鼠,取出瘤体,测量体积并称重比较。取出瘤体应用实时荧光定量PCR方法测定三组瘤体细胞中microRNA-27a的表达情况,确定实验组特异性抑制microRNA-27a表达有效。结果裸鼠皮下种植瘤生长曲线结果,实验组肿瘤生长速度明显高于阴性对照组和空白对照组,阴性对照组和空白对照组肿瘤的生长速度无明显差异。测量实验组、阴性对照组、空白对照组裸鼠体内种植瘤体积及质量差异有统计学意义(P<0.05)。每两组分别比较,实验组种植瘤平均体积及质量明显大于阴性对照组(P<0.05)和空白对照组(P<0.05),阴性对照组与空白对照组种植瘤平均体积及质量差异无统计学意义(P>0.05)。qRT-PCR检测三组裸鼠体内种植瘤细胞中microRNA-27a的相对表达水平,实验组中microRNA-27a表达水平明显低于阴性对照组(P<0.05)和空白对照组(P<0.05)。阴性对照组和空白对照组中microRNA-27a的表达水平差异无统计学意义(P>0.05)。结论下调microRNA-27a的表达水平可以促进肿瘤的生长,有望成为治疗宫颈癌的新靶点。

Objective To study the effect of microRNA-27 a on the growth of subcutaneously implanted tumors of cervical cancer in nude mice.Methods Nude mice subcutaneously implanted tumor models were established and randomly divided into three groups.The experimental group was subcutaneously implanted with intratumoral injection of microRNA-27 a-antagomir to specifically inhibit the expression of microRNA-27 a,the negative control group was subcutaneously injected with microRNA-27 a-antagomir-NC,and the blank control group was injected with PBS subcutaneously twice a week.The long diameter and short diameter of the subcutaneous implanted tumor were measured with a vernier caliper every three days,the tumor volume was calculated,and a growth curve was drew.One week after the last injection,the nude mice were sacrificed,the tumor was taken out,the volume was measured and weighed,and the three groups were compared.The tumor was taken out and quantitative real-time PCR was used to measure the expression of microRNA-27 a in the three groups of tumor cells,and to confirm that the experimental group specifically inhibits the expression of microRNA-27 a.Results The results of the growth curve of subcutaneously implanted tumors in nude mice showed that the tumor growth rate of the experimental group was significantly higher than that of the negative control group and the blank control group.There was no significant difference in the tumor growth rate of the negative control group and the blank control group.There were statistically significant differences in the volume and mass of tumor implanted in nude mice in the experimental group,negative control group,and blank control group(P<0.05).Comparing each group separately,the average volume and mass of tumor implanted in the experimental group was significantly greater than that of the negative control group(P<0.05)and the blank control group(P<0.05).There was no statistical difference between the negative control group and the blank control group(P>0.05).qRT-PCR results showed that the expression level of microRNA-27 a in the experimental group was significantly lower than the negative control group(P<0.05)and the blank control group(P<0.05).There was no significant difference in the expression level of microRNA-27 a between the negative control group and the blank control group(P>0.05).Conclusion Down-regulating the expression level of microRNA-27 a can promote the growth of tumors,which is expected to be a new target for the treatment of cervical cancer.