东北绵羊股骨骨密质组织形态学特征的研究

The research of histomorphological characteristics of domestic sheep femora

目的研究鉴别东北绵羊股骨骨密质组织学的形态特征。方法取50只东北2岁以上绵羊右后肢股骨中段,经过固定、脱钙、冰冻骨骼组织切片及硫堇苦味酸染色,在显微镜下观察、照相,输入电脑利用专业软件进行分析、统计数据。结果东北绵羊的股骨骨密质内、外环骨板与骨单位、间骨板分界比较清楚,内、外环骨板分层比较明显,其形态多呈连续状态,骨单位形态个体差异较大,骨陷窝数量较多,偶见到骨小梁。在50只东北绵羊股骨切片中,39个标本切片骨单位与间骨板分界清晰,骨单位呈椭圆和近圆形,排列多无规律。哈氏系统环层骨板分层比较清晰,骨细胞主要以呈层状排列的梭形形式存在。另11个标本切片骨单位与间骨板分界不清晰,骨单位数量较多,形态近似椭圆形,哈氏系统环层骨板分层不明显,骨单位整体排列有一定的走向性。结论东北绵羊的股骨骨密质组织学形态显示出其独有的特征,并反映出与其他动物的不同及其进化状态,对考古学、与其他动物的区别和法医学的种属鉴别具有重要意义,同时提供了生物进化研究新的观测指标。

Objective To study the histomorphological characteristics of sheep femora.Methods Take the middle fragment of 50 right domestic sheep femora to process histological slices.Observe slices under the microscope and send the image data into computer to conclude statistics.Results The stratification of the inner and outer circumferential lamellae are not distinct,existing in the form of fusiform bonny cells;Most osteons are circle or ovaland array irregularly;There are many bone lacunae and there is no bone trabecula.Conclusion The histomorphological features of domestic sheep femora are obviously.These data will help us in species identification and research of biological evolution.