高通量测序技术诊断婴幼儿广州管圆线虫嗜酸性脑膜脑炎2例

Diagnosis of angiostrongylus eosinophilic meningitis in infants by high-throughput sequencing:2 cases of reports

婴幼儿患广州管圆线虫嗜酸性脑膜脑炎(AEM)虽然罕见,但能造成严重后果,通过对2019年深圳市儿童医院神经内科收治的2例AEM患儿(<2岁)进行病例分析,探讨高通量测序对AEM的诊断价值。例1主要表现为间断发热、呕吐、精神差、前囟隆起。例2主要表现为间断发热、咳嗽、呕吐、抽搐。结合患儿外周血及脑脊液嗜酸性粒细胞增多,以及广州管圆线虫高通量测序及抗体检测阳性,最终确诊AEM。予阿苯达唑驱虫、小剂量泼尼松预防炎症反应后病情好转。婴幼儿AEM的临床特征不典型,高通量测序技术可协助诊断AEM。

Infants suffering from angiostrongylus eosinophilic meningitis(AEM)is rare,while AEM can cause severe consequences.The diagnostic value of high-throughput sequencing for AEM was studied by analyzing 2 AEM children(<2 years old)in the Department of Neurology,Shenzhen Children′s Hospital in 2019.Case 1 mainly pre-sented intermittent fever,vomiting,mental fatigue and bregma bulge.Case 2 mainly manifested intermittent fever,cough,vomiting and convulsion.Due to hypereosinophils in patients′peripheral blood and cerebrospinal fluid(CSF),and abundant DNA sequences from a cantonensis in CSF and positive antibody test,the patients were diagnosed with AEM.The patients were treated with albendazole to deworm,and small doses of methylprednisolone to reduce inflammation.The clinical characteristics of AEM infant are not typical,and high-throughput sequencing technology can assist the diagnosis of AEM.