线粒体氧化应激相关蛋白酶PRDX3在主动脉夹层中的表达及其意义

Expression and Significance of Mitochondrial Oxidative Stress-related Protease PRDX3 in Aortic Dissection

目的探讨主动脉夹层(AD)患者体内过氧化还原酶3的表达及其意义。方法选取2018年8月~2019年4月于武汉大学人民医院确诊为StanfordⅠ型主动脉夹层患者为AD组,另选接受心脏移植患者作为对照组。通过Western blot法和免疫组化染色检测主动脉夹层患者与正常对照组主动脉组织中PRDX3的表达差异;构建人主动脉血管平滑肌细胞(HASMC)氧化应激模型,提取细胞蛋白后使用Western blot法检测实验组与对照组的PRDX3表达差异;用0.3%β-异氨基丙腈酯(BAPN)构建SD大鼠主动脉夹层动物模型,取两组大鼠主动脉组织做石蜡包埋,标本切片后处理步骤及检测指标同人体标本。结果人体标本中Western blot法与免疫组化染色均发现,AD组PRDX3的表达较对照组正常主动脉明显增高;动物实验中免疫组化染色发现,AD组大鼠主动脉组织表达PRDX3显著增高;细胞实验中Western blot法结果显示随着H_(2)O_(2)浓度的升高PRDX3的表达也随之升高。结论PRDX3与线粒体氧化应激水平升高密切相关,PRDX3的升高说明在主动脉夹层中线粒体氧化应激水平升高。PRDX3表达的上调可能与夹层的发生、发展密切相关,给主动脉夹层的治疗提供新的靶点。

Objective To investigate the expression and significance of mitochondrial oxidative stress-related protease PRDX3 in aortic dissection.Methods Patients who were diagnosed with Stanford tape I with a parallel thoracic aortic dissection from August 2018 to April 2019 were enrolled in the AD group,and patients who were operated with heart transplantation were enrolled as donors.Western blot and immunohistochemical staining were used to detect the express differences of PRDX3 in aortic tissue of patients with aortic dissection and normal control group.We established the human aortic smooth muscle cells(HASMC)oxidative stress model,and extraced the cell protein to detect the express differences of PRDX3 between the hydrogen peroxide treated group and the control group by Western blot.An animal model of aortic dissection in SD rats was established by using 0.3%β-isoamino-propanonitrel(BAPN).The aortic tissues of the two groups of rats were embedded in paraffin.The post-treatment procedures and detection indexes of the specimens were the same as those of human specimens.Results In human samples,Western blot and immunohistochemistry showed that the expression of PRDX3 in aortic vessels of AD group was significantly higher than that in control group(P<0.05).In animal experiment,immunohistochemistry showed that the expression of PRDX3 was significantly increased in the aortic tissues of rats in AD group.Cell experiment also showed that the expression of PRDX3 in aorta of oxidative stress group was significantly higher than that of control group(P<0.05).Conclusion PRDX3 is closely related to mitochondrial oxidative stress.The increase of PRDX3 indicates elevated mitochondrial oxidative stress levels in aortic dissection.The up-regulation of PRDX3 expression may be closely related to the occurrence and development of aortic dissection,providing a new target for the treatment of aortic dissection in the future.