基于网络药理学探讨康妇炎胶囊治疗盆腔炎的分子作用机制

Molecular Mechanism of Kangfuyan Capsules in the Treatment of Pelvic Inflammatory Disease Based on Network Pharmacology

目的:借助网络药理学方法探讨康妇炎胶囊治疗盆腔炎(pelvic inflammatory disease,PID)的药效物质基础及其分子机制。方法:通过中药系统药理学数据库与分析平台和中药分子机制生物信息学分析工具数据库收集相应的化学成分及相关药物和疾病靶标,分析这些靶标之间的相互作用关系,构建核心靶标网络,并对核心靶标进行生物信息学分析,最后建立康妇炎胶囊11味中药-成分-靶标-通路网络。结果:经筛选获得康妇炎胶囊中的249种成分,共涉及460个关键靶标。对前150个核心靶标分析结果显示,这些核心靶标可能通过影响肿瘤坏死因子(TNF)信号通路、PI3K-Akt信号通路、趋化因子信号通路以及T细胞受体信号通路等炎症信号通路参与康妇炎胶囊治疗PID的过程,其中神经型一氧化氮合酶(NOS_(1))、CC趋化因子2(CCL2)、SRY相关高迁移率组蛋白9(Sox9)和前列腺素内过氧化物合酶(PTGS2)等4个重要的炎症介质可能是康妇炎胶囊治疗PID的重要靶标。结论:本研究为康妇炎胶囊治疗PID提供了理论依据,也为下一步体内外实验以及临床研究提供了重要参考。

OBJECTIVE:To analyze the pharmacodynamic material basis and molecular mechanism of Kangfuyan capsules in the treatment of pelvic inflammatory disease(PID)based on network pharmacology.METHODS:The corresponding chemical components and related drug and disease targets were collected through the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)and Bio-informatics Analysis Tool for Molecular Mechanism of Traditional Chinese Medicine(BATMAN-TCM)database,the interaction relationships between these targets were analyzed,the core target network was constructed,and the bioinformatics analysis of the core target was performed,and finally the herbal-component-target-pathway network of 11 kinds of ingredients of Kangfuyan capsules was established.RESULTS:Totally 249 kinds of components were screened out from Kangfuyan capsules,involving 460 key targets.The analysis on the former 150 core targets indicated that these core targets may participate in the treatment of PID by Kangwanyan capsules through influencing inflammatory signaling pathways including TNF signaling pathway,PI3K-Akt signaling pathway,chemokine signaling pathway and T cell receptor signaling pathway,among which the four important inflammatory mediators of NOS_(1),CCL2,Sox9 and PTGS2 may be the important targets of Kangfuyan capsules in the treatment of PID.CONCLUSIONS:This study provides a theoretical basis for the treatment of PID with Kangfuyan capsules,an important reference for the next in vivo and ex vivo experiments as well as clinical studies.