Inhibition effects of dexamethasone on matrix metalloproteinase in osteoarthritis cells

Objective:To investigate the inhibition effect of dexamethasone with different concentration gradients on the expression of MMP-1,-3,and-13 in a bone joint cell model of IL-1β-induced SW1353 cells.Methods:The SW1353 cells were placed in a culture medium containing 10^(1)-10^(8) nmol/L dexamethasone,and after 12 hours of culture,the appropriate intervention concentration range of dexamethasone was screened by the MTS for subsequent experiments.After SW1353 cells were induced with 10 ng/mL IL-1β,10^(1)-10^(7) nmol/L dexamethasone was given for intervention,and RT-PCR was used to detect the mRNA expression of MMP-1,-3,and-13.The protein was extracted after the intervention of 104 nmol/L dexamethasone,and western blot was used to detect the protein expression of MMP-1,-3,and-13.Results:MTS proliferation experiment results showed that 10^(1)-10^(7) nmol/L dexamethasone had no significant effect on SW1353 cell viability,while 10^(8) nmol/L dexamethasone had a significant inhibitory effect on SW1353(p<0.05).There was no obvious effect on cell viability after adding 10 ng/mL IL-1β.RT-PCR results indicated that the IL-1βgroup had a significant increase in the mRNA expression of MMP-1,-3,and-13 compared with the blank control group,and the difference was statistically significant(p<0.05).After intervention with 102-10^(7) nmol/L dexamethasone,MMP-1 mRNA expression decreased(p<0.05);after intervention with 103-10^(7) nmol/L dexamethasone,MMP-3 mRNA expression decreased(p<0.05);after intervention with 10-10^(7) nmol/L dexamethasone,the mRNA expression of MMP-13 decreased(p<0.05).The Western blot results showed that compared with the blank control group,the protein levels of MMP-1,-3,and-13 in the IL-1βgroup were significantly increased;the protein levels of MMP-1,-3,and-13 were significantly reduced after the intervention of 104 nmol/L dexamethasone.Conclusion:Dexamethasone can inhibit the expression of MMP1,MMP-3,and MMP-13 in the IL-1β-induced OA cell model at the mRNA and protein levels.