玉木耳的鉴定及其生长最佳营养条件研究

Identification of Auricularia cornea var.Li and Research on the Optimum Growth Conditions

以玉木耳菌株为研究对象,进行DNA测序鉴定确定其种源可靠性,并筛选其菌丝生长最佳营养条件,以期为玉木耳在贵州的引种示范提供基础理论依据。结果表明,供试菌株与毛木耳相似度达99%以上,为毛木耳下的一个分支。进一步研究发现,玉木耳菌丝生长条件并不苛刻,在25~35℃均能良好生长,其中最适温度为30℃,属于中高温型食用菌。其适宜碳源有可溶性淀粉、蔗糖和麦芽糖,可溶性淀粉为最佳;适宜氮源有豆粕粉、蛋白胨和酵母膏,豆粕粉最佳;挑选适宜碳氮源各2个,4因素3水平正交试验可得玉木耳菌丝生长最佳碳氮源浓度组合为可溶性淀粉为10.0 g,蔗糖10.0 g,豆粕粉2.5 g,蛋白胨1.5 g。

Taking the Auricularia cornea var.Li strain provided by the Guizhou Province Edible Fungus Engineering Technology Research Center as the research object,DNA sequencing was performed to determine the reliability of its provenance,and the optimal nutrient conditions for its mycelial growth were screened.It hopes to provide a basic theoretical basis for the introduction and demonstration of Auricularia cornea var.Li in Guizhou.The identification shows that the similarity between the tested strain and Auricularia polytricha was more than 99%,so it is a branch under Auricularia polytricha.Further research shows that the growth conditions of Auricularia cornea var.Li hyphae are not harsh.It can grow well at 25-35℃,among which the optimum temperature is 30℃,which is a medium-high temperature edible fungus.Its suitable carbon sources are soluble starch,sucrose and maltose,and soluble starch is the best.Its suitable nitrogen sources are soybean meal powder,peptone and yeast extract,and soybean meal powder is the best.In this paper,two suitable carbon and nitrogen sources were selected,and a 4-factor 3-level orthogonal experiment was carried out.In the end,the best combination of carbon and nitrogen source concentration for the growth of Auricularia cornea var.Li mycelium is 10.0 g soluble starch,10 g sucrose,2.5 g soybean meal powder,and 1.5 g peptone.