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渔用氧化剂对水源水和池塘水中磺胺类抗性基因sul1的去除作用 被引量:3

Removal of sulphonamide resistance sul1 gene in water source and pond water by fishery oxidants in aquaculture
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摘要 为探讨利用渔用氧化剂去除养殖水体环境中的抗生素抗性基因(Antibiotic resistant genes,ARGs)并控制其传播的可行性,该研究以磺胺类抗性基因sul1作为目标抗性基因,选用次氯酸钠(NaClO)、二溴海因(C_(5)H_(6)Br_(2)N_(2)O_(2))和高锰酸钾(KMnO_(4))3种养殖中常用的渔用氧化剂,运用实时荧光定量PCR技术,分析目标渔用氧化剂对水源水和池塘水体中ARGs的去除效果。结果显示,测试的水源水和池塘水体中的sul1浓度均处于较高水平,分别达到了10^(5)和10^(6)拷贝·mL^(−1),而且微生物的细胞内sul1是其主要存在形式;其次,NaClO对各实验组中的sul1均呈现较强的去除效果,C_(5)H_(6)Br_(2)N_(2)O_(2)仅对水源水中细胞内和细胞外的sul1具有较强的去除作用,KMnO_(4)仅对不同水体中的细胞外sul1具有较强的去除作用。结果表明,3种目标渔用氧化剂中,NaClO对水源水和池塘水体中ARGs的去除效果最好,科学应用NaClO有利于去除水产养殖水环境中的ARGs,进而防控其在养殖过程中的传播。 To explore the feasibility of using fishery oxidants to eliminate antibiotic resistant genes(ARGs)in aquaculture water and to control their spread,we studied the effects on ARG removal of three commonly used fishery oxidants(Sodium hypochlorite,dibromohydantoin and potassium permanganate)in water source and pond water by real-time fluorescent quantitative PCR.Results show that the concentration of sul1 gene was high in the water source and pond water(10^(5)and 10^(6)copies·mL^(−1),respectively),and the intracellular sul1 gene was the predominant form.Additionally,sodium hypochlorite had a strong effect on sul1 gene removal among all the experimental groups,and dibromohydantoin only showed a strong removal effect on sul1 gene in the water source.Potassium permanganate only showed a strong effect on the removal of extracellular sul1 gene in both water source and pond water.The results indicate that sodium hypochlorite has the best effect on ARG removal in both water source and pond water for these three common used fishery oxidants.Scientific application of sodium hypochlorite is beneficial to ARG removal and prevention in aquaticulture water.
作者 赵晓雨 苏浩昌 徐煜 徐武杰 胡晓娟 文国樑 曹煜成 余招龙 ZHAO Xiaoyu;SU Haochang;XU Yu;XU Wujie;HU Xiaojuan;WEN Guoliang;CAO Yucheng;YU Zhaolong(College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China;South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Resources Exploitation&Utilization,Ministry of Agriculture and Rural Affairs/Guangdong Provincial Key Laboratory of Fishery Ecology and Environment,Guangzhou 510300,China;Shenzhen Base of South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shenzhen 518121,China;Guangdong Guanli Marine Biology Co.,Ltd.,Maoming 525400,China)
出处 《南方水产科学》 CAS CSCD 北大核心 2021年第3期46-53,共8页 South China Fisheries Science
基金 国家重点研发计划项目(2019YFD0900402) 中国水产科学研究院基本科研业务费专项资金(2020TD54) 财政部和农业农村部国家现代农业产业技术体系资助(CARS-48) 广东省现代农业产业技术体系创新团队建设专项资金(2019KJ149)。
关键词 抗生素抗性基因 渔用氧化剂 次氯酸钠 二溴海因 高锰酸钾 Antibiotic resistance genes Fishery oxidants Sodium hypochlorite Dibromohydantoin Potassium permanganate
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