上皮性卵巢癌患者血清miR-222-3p、HE4及CA125的表达及其临床意义

Expression and Clinical Significance of MiR-222-3p,HE4 and CA 125 in Serum of Epithelial Ovarian Cancer

目的探讨上皮性卵巢癌(EOC)患者血清miR-222-3p、血清人附睾蛋白4(HE4)及糖类抗原125(CA125)的表达及其临床意义。方法选取本院收治的118例EOC患者,105例上皮性卵巢良性肿瘤患者和60例健康女性作为研究对象,采用实时定量PCR法检测miR-222-3p表达水平,化学发光法测定血清HE4及CA125水平。应用受试者工作特征(ROC)曲线分析血清miR-222-3p、HE4及CA125水平对EOC的诊断价值。Pearson相关分析EOC患者血清miR-222-3p表达水平与HE4及CA125的相关性。结果EOC组血清miR-222-3p(1.62±0.74 vs 0.84±0.35,0.73±0.24)、HE4(346.20±58.72 pmol/L vs 53.46±11.63 pmol/L,48.60±9.75 pmol/L)及CA125(307.60±94.25 U/mL vs 26.83±11.56 U/mL,20.72±9.40 U/mL)水平均明显高于良性组和对照组(均P<0.001)。EOC患者血清miR-222-3p、HE4及CA125表达与临床分期、分化程度及淋巴结转移有关(P<0.01)。ROC曲线显示,血清miR-222-3p、HE4及CA125水平诊断EOC的最佳截断值分别为1.16、187.50 pmol/L、162.80 U/mL,三项联合诊断EOC的曲线下面积(0.948,95%CI:0.887~0.995)最大,其敏感度和特异度为96.8%和89.5%。相关分析显示,EOC患者血清miR-222-3p表达水平与HE4及CA125均呈正相关(r=0.847,P<0.001,r=0.793,P<0.001)。结论EOC患者血清miR-222-3p、HE4及CA125水平明显升高,三项联合检测对EOC的诊断具有较高的价值。

Objective To investigate the expression of serum miR-222-3p,human epididymal protein 4(HE4)and carbohydrate antigen 125(CA125)in epithelial ovarian cancer(EOC)and its clinical significance.Methods A total of 118 EOC patients,105 in the benign group and 60 in the healthy control group were selected in our hospital.The expression of serum miR-222-3p was detected by real-time quantitative PCR,and the levels of HE4 and CA125 were detected by chemiluminescence.The diagnostic value of serum miR-222-3p,HE4 and CA125 levels to EOC were analyzed by ROC curve.Pearson correlation analysis showed that the expression of miR-222-3p in EOC patients was correlated with HE4 and CA125.Results The levels of serum miR-222-3p(1.62±0.74 vs 0.84±0.35,0.73±0.24),HE4(346.20±58.72 pmol/L vs 53.46±11.63 pmol/L,48.60±9.75 pmol/L)and CA125(307.60±94.25 U/mL vs 26.83±11.56 U/mL,20.72±9.40 U/mL)in EOC group were significantly higher than those in benign group and control group(P<0.001).ROC curve showed that the best cutoff values of serum miR-222-3p,HE4 and CA 125 were 1.16,187.50 pmol/L and 162.80 U/mL,respectively.The area under the curve(0.948,95%CI:0.887~0.995)of the three combined diagnosis of EOC was the largest,and its sensitivity and specificity were 96.8%and 89.5%.Correlation analysis showed that the expression of miR-222-3p in EOC patients were positively correlated with HE4 and CA125(r=0.847,P<0.001,r=0.793,P<0.001).Conclusion The levels of serum miR222-3p,HE4 and CA125 were significantly increased in EOC patients,and the three combined tests were of high value in the diagnosis of EOC.