羊草幼穗组织培养与再生体系建立的研究

Establishment of Tissue Culture and Regeneration System of Young Spikes of Leymus chinensis

为了优化羊草组织培养与再生体系,本试验以6个羊草[Leymus chinensis(Trin.)Tzvel.]新品系的幼穗为外植体,使用添加不同植物激素的MS培养基对羊草幼穗进行愈伤组织诱导、继代、分化等培养,建立高效再生体系。结果表明:诱导培养基中添加2,4-D激素浓度为5.0 mg·L-1时,6个羊草新品系幼穗的诱导率均较高,其中Lc-6出愈率最高,为87.5%,其次是Lc-4,诱导率为81.5%;6个品系愈伤组织通过固-液-固(MS2-MSL2-MS2)交替继代培养,愈伤组织结构与状态得到明显改善,愈伤由疏松变得致密,褐化现象明显减少,愈伤变为乳白色颗粒状,Lc-6和Lc-4的愈伤状态优于其他品系;6个品系愈伤组织在BNKT分化培养基上的分化率显著高于其他2种培养基(P<0.05),Lc-6分化率最高,为41.25%;6个品系移栽后组培苗成活率均为100%。

In order to optimize tissue culture and regeneration system of Leymus chinensis,six young panicles of Leymus chinensis were used as explants,and MS medium supplemented with different plant hormones was used to induce subculture differentiation of young panicles of Leymus chinensis,so as to establish an efficient regeneration system.The results showed that when the concentration of 2,4-D hormone was 5.0 mg·L-1 in the induction medium,the induction rate of young panicles of the six new lines of Leymus chinensis were all high.The callus rate of Lc-6 was the highest(87.5%),followed by Lc-4(81.5%).The callus structure and state of the six strains were significantly improved by solid-liquid-solid(MS2-MSL2-MS2)subculture,the callus changed from loose to compact,the browning was significantly reduced,and the callus turned to milky white granules.The callus state of Lc-6 and Lc-4 was better than that of the other strains.The callus differentiation rate of the six strains on BNKT medium was significantly higher than that of the other two media(P<0.05),the differentiation rate of Lc-6 was the highest(41.25);After transplantation,the survival rate of tissue culture seedlings of six strains was 100%.