基于网络药理学探讨肛肠瘙痒洗剂治疗肛周湿疹的作用机制

Study on the Mechanism of Anal Pruritus Lotion in the Treatment of Perianal Eczema based on Network Pharmacology

目的:基于网络药理学方法初步探讨肛肠瘙痒洗剂治疗肛周湿疹的机制。方法:应用TCMSP数据库,根据DL≥0.18,ALogP<0.5对成分信息进行筛选后,检索药物相关靶点,共获得药物靶点258个。在GeneCards数据库中查找“湿疹”疾病相关基因,共获得相关基因2736个。药物和疾病共有靶点114个。使用String数据库获得蛋白-蛋白相互作用(PPI)关系,用Cytoscape软件进行可视化。根据蛋白相互作用的“degree”值,获得关键作用靶点,采用R软件中的“Bioconductor”安装包对靶点进行GO及KEGG通路分析。结果:网络分析结果表明,肛肠瘙痒洗剂主要通过细胞因子及受体诱导免疫激活,可能通过调节IL-17信号通路、TNF信号通路、Toll样受体信号通路、Th1及Th2细胞分化、JAK-STAT信号通路、Th17细胞分化等信号通路来发挥其治疗作用。结论:本研究综合应用网络药理学的方法预测出肛肠瘙痒洗剂治疗湿疹的作用机制和分子靶点,为其进一步实验研究鉴定基础。

Objective Preliminary study on the mechanism of anal pruritus lotion in the treatment of perianal eczema based on network pharmacology.Methods The TCMSP database was used.After screening the component information according to DL≥0.18 and ALogP<0.5,searching for drug-related targets,a total of 258 drug targets were obtained.Searching for genes related to“eczema”disease in the GeneCards database,a total of 2736 related genes were obtained.There are 114 targets for drugs and diseases.The protein protein interaction(PPI)relationship was obtained by string database and visualized by Cytoscape software.According to the“degree”value of protein interaction,the key targets were obtained,which suggested that the anal pruritus lotion may participate in cytokine-mediated cell signal transduction to initiate the effect.The“Bioconductor”installation package in R software was used to analyze the GO and KEGG pathways of the target.Results Network analysis results showed that the anal pruritus lotion mainly induced immune activation through cytokines and receptors,and might exert its biological activity through signal pathways such as IL-17,TNF,Toll receptor,JAK-STAT,Th1 and Th2 cell differentiation.Conclusion This study comprehensively applied the method of network pharmacology to predict the mechanism and molecular targets of anal pruritus lotion in the treatment of eczema,which is the basis for further experimental research and identification.