基于网络公开测序数据的K326烟草线粒体基因组RNA编辑位点的鉴定与分析

Identification and analysis of K326 tobacco mitochondrial genome RNA editing sites based on public online sequencing data

为探索RNA编辑位点在烟草线粒体中的分布和功能,通过分析烟草根、花、叶的基因组及转录组测序数据,鉴定了烟草线粒体中从胞嘧啶(C)转换为尿嘧啶(U)的RNA编辑位点。结果显示,在烟草线粒体上共发现了4212个RNA编辑位点,其中30.2%的RNA编辑位点位于蛋白编码基因(rps3、mat-R和ccmFN为RNA编辑位点最多的3个蛋白编码基因),62个RNA编辑位点属于产生的无义突变或使终止密码子丢失的位点。对根、花、叶的RNA编辑位点进行比较,叶的位点最多,为2923个。对这3种器官的特异无义突变RNA编辑位点进行分析,发现其所在的基因参与了氧化呼吸链的电子传递、蛋白合成等过程。同时553个RNA编辑位点位于开放阅读框基因上,说明这些开放阅读框基因可能也参与了线粒体某些功能的完成。

To investigate the distributions and functions of RNA editing sites in tobacco mitochondria,the genome and transcriptome sequencing data from tobacco roots,flowers and leaves were analyzed to identify the RNA editing sites that converted cytosine(C)to uracil(U)in tobacco mitochondria.The results showed that 4212 RNA editing sites in tobacco mitochondria were identified,wherein 30.2%editing sites were located in protein-coding genes and rps3,mat-R and ccmFN were the protein-coding genes with the highest number of editing sites.There were 62 RNA editing sites belonging to nonsense mutations or mutations that resulted in stop codon loss.Comparing the number of the editing sites in tobacco roots,flowers and leaves,the leaves had the highest number(2923)of editing sites.The analysis of the RNA editing sites for nonsense mutations in the 3 organs suggested that the genes where these sites were located in were involved in the biological process of electron transport in the oxidative respiratory chain and protein synthesis.In total,553 RNA editing sites were located in open reading frame genes,which indicated that these open reading frame genes might be involved in some functions of mitochondria.