摘要
Mud crab(Scylla paramamosain)is one of the most consumed crabs in China.Generally,it is believed that dead mud crabs are not edible.Herein,we investigated the postmortem metabolite profile changes of the muscle and hepatopancreas of mud crab associated with storage method using nuclear magnetic resonance-based metabolomics coupled with multivariate data analysis.Our results showed that postmortem changes of trimethylamine,organic acids(lactate,formate,and succinate),as well as ATP and its breakdown products are closely affected by the storage method.These metabolites can be used as indicators for monitoring loss of crab freshness.Furthermore,dead mud crabs preserved an edible quality at least for 4 h at room temperature,12h on ice,and 16 d at−20℃,strongly suggesting that dead crab consumption quality should be determined by freshness.They shouldn’t be discarded without considering the effects of different storage conditions on their freshness.
基金
Financial support from the 2025 Technological Innova-tion for Ningbo(No.2019B10010)
China Agriculture Re-search System-CARS48
K.C.Wong Magna Fund in Ningbo University
the Special research funding from the Marine Biotechnology and Marine Engineering Dis cipline Group in Ningbo University(No.422004582)are greatly acknowledged.
