基于谱效关系的肉桂改善肾阳虚作用的质量标志物(Q-Marker)研究

Quality marker study of Cinnamomi Cortex on improving kidney-yang deficiency based on spectrum-effect relationship

目的建立基于谱效关系的肉桂(Cinnamomum cassia的干燥树皮)改善肾阳虚作用的质量标志物(quality marker,Q-Marker)研究方法,为其药效物质基础及质量控制研究提供依据。方法采用高效液相色谱法,建立不同产地肉桂水煎液及甲醇提取物的指纹图谱。采用氢化可的松建立小鼠肾阳虚症模型,以体质量增长率、三碘甲状腺氨酸(triiodothyronine,T3)、四碘甲状腺氨酸(tetraiodothyronine,T4)、促肾上腺皮质激素(adrenal cortical hormone,ACTH)、肾脏指数、睾丸指数作为指标评价不同产地肉桂的改善肾阳虚作用。通过灰色关联度分析构建色谱峰与药效指标的相关性,筛选Q-Marker。结果在改善肾阳虚方面,肉桂水煎液中肉桂酸为关联度较高的成分(r>0.74);肉桂甲醇提取物中肉桂醛、肉桂酸关联度最高(r>0.80),邻甲氧基肉桂醛关联度较高(r>0.72),初步确认了肉桂醛、肉桂酸、邻甲氧基肉桂醛为肉桂改善肾阳虚的药效物质基础即Q-Marker。结论通过谱效关系研究,探讨了肉桂改善肾阳虚作用的Q-Marker,可为肉桂改善肾阳虚作用的药效物质基础及质量标准研究提供依据。

Objective To establish a quality marker research method of Rougui(Cinnamomum cassia)based on the spectrum-effect relationship study on its improving kidney yang deficiency,so as to provide some basis for its pharmacodynamic material basis and quality control.Methods HPLC was used to establish the fingerprints of water and methanol extract of different batches of C.cassia.Kidney-yang deficiency mice model was established by intragastric administration of hydrocortisone,and the improving effects of C.cassia on kidney-yang deficiency were evaluated by body weight growth rate,T3,T4,ACTH,kidney coefficient and testis coefficient.The correlation between chromatographic peaks and pharmacodynamic indexes was constructed by grey correlation analysis to screen quality markers.Results Cinnamic acid in the water extract of C.cassia was the component with high correlation(r>0.74),and cinnamaldehyde and cinnamic acid in the methanol extract had the highest correlation(r>0.80)and o-methoxy cinnamaldehyde had high correlation(r>0.72).It was preliminarily confirmed that the above-mentioned three components were the effective improving kidney-yang deficiency substances of C.cassia.Conclusion The results of this study can provide evidence for the effective substance and quality markers study of C.cassia based on its improving kidney-yang deficiency effect.