基于肉桂质量标志物(Q-Marker)预测的质量控制研究

Study on quality control of Cinnamomum cassia based on prediction of Q-Marker

目的建立一种基于肉桂Cinnamomum cassia质量标志物(quality marker,Q-Marker)预测的质量控制方法,并运用化学计量学综合分析不同产地肉桂质量差异。方法采用高效液相色谱法,通过同时测定肉桂中肉桂醛、香豆素、肉桂醇、肉桂酸及邻甲氧基肉桂醛5种Q-Marker含量,建立肉桂药材的指纹图谱,采用聚类分析、主成分分析等化学计量学手段对27批肉桂药材质量进行评价。结果建立了一测多评法,肉桂醇、肉桂酸及邻甲氧基肉桂醛与内参物肉桂醛的相对校正因子分别为0.1357、0.2115、1.5927;建立了不同产地27批肉桂的HPLC指纹图谱及共有模式,并进行聚类分析、主成分分析和正交偏最小二乘判别分析。结果显示,27批不同产地肉桂可聚为5类,肉桂醛、香豆素是体现3个主产地间样品差异的主要标志性成分,并提示越南产的肉桂成分含量与中国广东、广西产的肉桂存在差异。结论建立的一测多评法能准确、简便地测定肉桂中Q-Marker的含量,不同产地的肉桂药材质量存在一定的差异性。为肉桂质量控制提供了更为科学、全面的依据和基础。

Objective To establish a quantitative analysis of Cinnamomum cassia based on the prediction of quality marker(Q-Marker),and to analyze the quality differences of C.cassia from different areas by chemometrics.Methods An HPLC method were developed for the determination of five quality markers including cinnamaldehyde,coumarin,cinnamyl alcohol,cinnamic acid,and o-methoxy cinnamic aldehyde in C.cassia in order to establish the fingerprints of C.cassia.The quality of 27 batches of C.cassia was evaluated by cluster analysis,principal component analysis,and other chemometrics methods.Results The relative correlation factors of cinnamyl alcohol,cinnamic acid,and O-methoxy cinnamic aldehyde were 0.1357,0.2115,and 1.5927.The HPLC fingerprints and common patterns of 27 batches of C.cassia from different origins were established,and cluster analysis,principal component analysis and orthogonal partial least squares discriminant analysis were carried out.The results showed that 27 batches of C.cassia can be clustered into five groups.Cinnamaldehyde and coumarin were the main symbolic components reflecting the difference among the three main producing areas.It was suggested that the content of C.cassia in Vietnam was different from that of C.cassia produced in Guangdong and Guangxi Provinces of China.Conclusion The established QAMS can accurately and easily determine the content of Q-Markers in C.cassia.There are certain differences in the quality of C.cassia from different origins.It provides a more scientific and comprehensive basis for the quality control of C.cassia.