摘要
目的观察蒲公英多糖对急性肾损伤(AKI)大鼠的保护作用。方法通过腹腔注射140 mg·kg^(-1)庆大霉素构建AKI模型,并随机分为模型组和低、高剂量实验组,每组18只;另选取18只雄性Wistar大鼠作为正常组。造模结束24 h后,低、高剂量实验组按0.01 mL·g^(-1)·d^(-1)的给药剂量分别灌胃给予50和400 mg·kg^(-1)蒲公英多糖;正常组和模型组均灌胃给予等量0.9%NaCl。4组大鼠每天给药1次,连续给药7 d。用WST-8法检测大鼠肾组织中超氧化物歧化酶(SOD)的活性,用实时荧光定量聚合酶链反应法检测各组大鼠肾组织中SOD-1和白细胞介素-10(IL^(-1)0)mRNA的表达水平。结果给药7 d后,低、高剂量实验组和模型组、正常组的SOD分别为(44.19±6.48),(65.49±7.31),(35.84±6.29)和(79.32±7.57)U·mg^(-1),SOD-1 mRNA分别为0.23±0.11,0.45±0.09,0.16±0.07和0.48±0.11,IL^(-1)0 mRNA分别为0.21±0.07,0.41±0.10,0.17±0.08和0.47±0.11,低、高剂量实验组的上述指标与模型组比较,差异均有统计学意义(均P<0.05)。结论蒲公英多糖对急性肾损伤大鼠有保护作用,其机制可能与减轻炎症及氧化应激反应有关。
Objective To investigate the protective effect and mechanism of dandelion polysaccharide on acute kidney injury rats.Methods The rats were injected intraperitoneally with 140 mg·kg^(-1)gentamicin to create a model,and all of them were divided into model group,experimental-L group and experimental-H group,with 18 cases per group.Then 18 Wistar rats were severed as normal group.The experimental-L group and experimental-H group were given 50 and 400 mg·kg^(-1)dandelion polysaccharide,respectively,normal group and model group were given equal volume of saline.4 groups of rats were administered once a day for 7 days.The activity of superoxide dismutase(SOD)in kidney tissue of rats in each group was detected by WST-8.The levels of SOD-1 mRNA and interleukin-10(IL-10)mRNA in kidney tissue of rats in each group were detected by real-time polymerase chain reaction,the protein levels of SOD-1 and IL-10 in kidney tissue of rats in each group were detected by Western blot.Results After administration of 7 days,the main indexes of experimental-L,experimental-H,model and normal groups were compared:the levels of SOD were(44.19±6.48),(65.49±7.31),(35.84±6.29)and(79.32±7.57)U·mg^(-1),the expression levels of SOD-1 mRNA were 0.23±0.11,0.45±0.09,0.16±0.07 and 0.48±0.11,the expression levels of IL-10 mRNA were 0.21±0.07,0.41±0.10,0.17±0.08 and 0.47±0.11,there were statistically significant differences between experimental-L,-H groups and model group(all P<0.05).Conclusion Taraxacum polysaccharide could protect the kidney of rats with acute renal injury and its mechanism may be related to alleviating inflammatory reaction and oxidative stress.
作者
陈献宇
刘圣君
金玉杰
CHEN Xian-Yu;LIU Sheng-jun;JIN Yu-Jie(Graduate School,Hebei North University,Zhangjiakou 075000,Hebei Province,China;Department of Nephrology,The First Affiliated Hospital of Hebei North University,Zhangjiakou 075000,Hebei Province,China)
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2021年第11期1367-1370,共4页
The Chinese Journal of Clinical Pharmacology
基金
河北省医学科学重点课题计划资助项目(20110540)。
关键词
蒲公英多糖
急性肾损伤
炎症反应
氧化应激反应
taraxacum polysaccharide
acute kidney injury
inflammatory reaction
oxidative stress