鱼腥草内生菌Aspergillus sp.GZWMJZ-636次级代谢产物研究

Secondary metabolites of endophytic fungus Aspergillus sp.GZWMJZ-636 from Houttuynia cordata

目的研究鱼腥草内生真菌Aspergillus sp.GZWMJZ-636的次级代谢产物和抗植物病原菌活性。方法采用硅胶柱色谱、高效液相色谱等方法对化合物进行分离纯化,采用核磁共振谱、质谱和Marfey水解等方法确定化合物的化学结构,采用平板法测试化合物的抗植物病原菌活性。结果从鱼腥草内生真菌Aspergillus sp.GZWMJZ-636的发酵产物中分离鉴定了6个化合物,分别为18-氧代曲普鲁斯太汀B(1)、1,2-裂环-杀锥曲菌素(2)、常现青霉素(3)、短密青霉菌胺F(4)、特来孢菌嗪D(5)、去甲氧基烟曲霉素C(6)。在50μg/mL质量浓度下,化合物2和3对小麦赤霉病菌的抑制率分别为21.4%和20.0%,对辣椒枯萎病菌抑制率分别为18.0%和20.9%。结论化合物1为新的吲哚二酮哌嗪生物碱,鱼腥草内生菌可代谢产生抗植物病原菌活性的化合物。

Objective To investigate the secondary metabolites and anti-phytopathogenic activities of the endophytic fungus Aspergillus sp.GZWMJZ-636 from Houttuynia cordata.Methods The secondary metabolites were isolated and purified by column chromatography over the silica gel and HPLC.Their structures were identified by the spectroscopic analysis and Marfey analysis.The anti-phytopathogenic activities were assayed by plate method.Result One new compound,named 18-oxotryprostatin B(1)along with five known compounds,1,2-seco-trypacidin(2),questin(3),brevianamide F(4),terezine D(5),and demethoxyfumitremorgin C(6)were identified.At the concentration of 50μg/mL,compounds 2 and 3 showed anti-phytopathogenic activities against Fusarium graminearum with the inhibition rates 21.4%and 20.0%,and the inhibition rates 18.0%and 20.9%against Fusarium oxysporum,respectively.Conclusion Compound 1 is a new indole diketopiperazine.Endophytes from H.cordata can produce active compounds against plant pathogens.