幽门螺杆菌感染慢性萎缩性胃炎易感性与miR-429基因rs7521584多态性

Relationship between susceptibility of chronic atrophic gastritis caused by Helicobacter pylori infection and polymorphism of miR-429 gene rs7521584

目的探讨miR-429基因rs7521584多态性与幽门螺杆菌(Hp)感染慢性萎缩性胃炎(CAG)易感性及严重程度关系。方法选择2017年5月-2019年5月江南大学附属医院消化内科收治的CAG患者70例作为CAG组,选择同期医院进行体检的健康人员70名作为对照组,提取研究对象全血DNA后,采用聚合酶链式扩增反应(PCR)和Sagner测序法对外周血中miR-429基因rs7521584位点单核苷酸多态性(SNP)进行检测。对CAG组患者肠化生、萎缩程度、临床症状严重程度进行评分,比较两组及研究组组内血清胃蛋白酶原Ⅰ(PepsinogenⅠ,PGⅠ)、胃蛋白酶原Ⅱ(PepsinogenⅡ,PGⅡ)和胃泌素(Gastrin,G-17)水平。结果两组患者miR-429基因rs7521584位点基因型分布经检验符合H-W平衡。两组miR-429基因rs7521584位点基因型分布差异具有统计学意义(P<0.05),CAG组T等位基因频率高于非CAG组,差异具有统计学意义(P<0.05);Logistic回归分析显示miR-429基因rs7521584位点T等位基因携带者人群CAG发病风险高于GG基因型携带人群(OR=2.15,95%CI=1.57~2.66,P=0.024),对比G与T等位基因分布频率,发现相对于G等位基因,T等位基因与CAG发病风险存在明确的相关性(OR=1.84,95%CI=1.33~2.49,P=0.030);CAG组患者miR-429基因rs7521584位点不同基因Hp阳性率差异具有统计学意义(P<0.05),TT型CAG患者Hp阳性率最高;CAG组患者miR-429基因rs7521584位点不同基因严重程度评分及血清学指标比较差异无统计学意义。结论miR-429基因rs7521584位点多态性与CAG发病及Hp感染阳性有关,T基因可能为易感基因,但与疾病严重程度和胃功能指标无关。

OBJECTIVE To explore the relationship between the polymorphism of miR-429 gene rs7521584 and the susceptibility and severity of chronic atrophic gastritis(CAG)caused by Helicobacter pylori infection.METHODS Totally 70 CAG patients who were treated in gastroenterology the department of the Affiliated Hospital of Jiangnan University from May 2017 to May 2019 were assigned as the CAG group,meanwhile,70 healthy people who received physical examination were set as the control group.The whole blood DNA were extracted from the study subjects,the single nucleotide polymorphism(SNP)of miR-429 gene rs7521584 locus in peripheral blood was detected by using polymerase chain reaction(PCR)and Sagner sequencing method.The intestinal metaplasia,degree of atrophy and severity of clinical symptoms were scored for the CAG patients,and the levels of serum pepsinogen I(PGⅠ),pepsinogenⅡ(PGⅡ)and gastrin(G-17)were compared between the two groups of patients and among the patients in the study group.RESULTS The genotype distribution of miR-429 gene rs7521584 locus of the two groups of patients was tested to meet H-W balance.There was significant difference in the genotype distribution at rs7521584 locus of miR-429 gene between the two groups(P<0.05).The frequency of T allele of the CAG group was significantly higher than that of the non-CAG group(P<0.05).Logistic regression analysis showed that the people who carried the T allele at rs7521584 of miR-429 gene were at higher risk of CAG than those who carried GG genotype(OR=2.15,95%CI=1.57~2.66,P=0.024).As compared with distribution frequency of G allele with T allele,T allele showed significantly association with the risk of CAG(OR=1.84,95%CI=1.33~2.49,P=0.030).There was significant difference in the positive rate of Hp among different genotypes at miR-429 gene rs7521584 loci of the patients in the CAG group(P<0.05),the positive rate of Hp of the CAG patients with genotype TT was the highest.There were no significant differences in the score of severity and serological indexes among the genotypes at miR-429 gene rs7521584 locus of the CAG patients.CONCLUSION The polymorphism at rs7521584 locus of miR-429 gene is related to the onset of CAG and Hp infection.T gene may be a susceptible gene,but it has nothing to do with the severity of disease severity or the gastric function indexes.