降钙素原与Toll样受体mRNA和核因子κB在感染性腹泻患者中的表达及意义

Expression of procalcitonin,Toll-like receptor mRNA and nuclear factorκB in patients with infectious diarrhea and its significance

目的探究降钙素原(PCT)、Toll样受体(TLR)mRNA、核因子κB(NF-κB)在感染性腹泻患者中的表达及意义。方法选取2019年1月-2020年1月河南省人民医院收治的感染性腹泻患者121例作为感染性腹泻组,同期选取80名健康志愿者作为对照组。采用双抗体夹心酶联免疫吸附试验法(ELISA)检测血清PCT、NF-κB水平,采用荧光定量PCR法(RT-PCR)检测所有研究对象血TLR2 mRNA、TLR4 mRNA表达量。分析在感染性腹泻患者中的表达及意义。结果与对照组相比,感染性腹泻组PCT、NF-κB水平及TLR2 mRNA、TLR4 mRNA表达量升高(t=15.930、18.240、12.930、13.480,P均<0.001);随着病程的延长,感染性腹泻患者PCT、NF-κB水平及TLR2 mRNA、TLR4 mRNA表达量升高,表现为慢性期高于持续性期、持续性期高于急性期,差异具有统计学意义(t=20.700、17.175、14.664、7.196,P均<0.001)。PCT、NF-κB水平及TLR2 mRNA、TLR4 mRNA表达量与感染性腹泻组患者疾病类型无关;与病毒性腹泻相比,细菌性腹泻患者PCT、NF-κB水平、TLR2 mRNA、TLR4 mRNA表达量升高,差异具有统计学意义(t=26.544、10.151、5.505、6.626,P均<0.001)。结论感染性腹泻患者存在PCT、NF-κB水平及TLR2 mRNA、TLR4 mRNA表达量升高现象,与病程、感染源相关,可能参与疾病的发生。

OBJECTIVE To explore the expression of procalcitonin(PCT),Toll-like receptor(TLR)mRNA and nuclear factorκB(NF-κB)in patients with infectious diarrhea and analyze the significance.METHODS A total of 121 patients with infectious diarrhea who were treated in Henan Provincial People′s Hospital from Jan 2019 to Jan 2020 were assigned as the infectious diarrhea group,meanwhile,80 healthy volunteers were chosen as the control group.The levels of serum PCT and NF-κB were detected by using double antibody sandwich enzyme-linked immunosorbent assay(ELISA),the expression levels of TLR2 mRNA and TLR4 mRNA were determined by means of fluorescence quantitative PCR,and the significance of the expression in the infection diarrhea patients was observed.RESULTS The levels of PCT and NF-κB as well as the expression levels of TLR2 mRNA and TLR4 mRNA were significantly higher in the infectious diarrhea group than in the control group(t=15.930,18.240,12.930,13.480,all P<0.001).With the extension of disease course,the levels of PCT and NF-κB as well as the expression levels of TLR2 mRNA and TLR4 mRNA of the patients with infectious diarrhea were elevated,the levels of above indexes of the chronic phase patients were significantly higher than those of the persistent phase patients,the levels of above indexes of the persistent phase patients were significantly higher than those of the acute phase patients(t=20.700,17.175,14.664,7.196,all P<0.001).The levels of PCT and NF-κB as well as the expression levels of TLR2 mRNA and TLR4 mRNA of the patients with infectious diarrhea were not associated with the type of disease;the levels of PCT and NF-κB as well as the expression levels of TLR2 mRNA and TLR4 mRNA were significantly higher in the patients with bacterial diarrhea than in the patients with viral diarrhea(t=26.544,10.151,5.505,6.626,all P<0.001).CONCLUSION The levels of PCT and NF-κB as well as the expression levels of TLR2 mRNA and TLR4 mRNA are elevated in the patients with infectious diarrhea,which is associated with the disease course and source of infection and may participate in the occurrence of the disease.