TLRs-NF-κΒ信号通路在重症肺炎儿童中的作用分析

Role of TLRs-NF-κΒ Signaling Pathway in Children with Severe Pneumonia

【目的】探讨Toll样受体-核转录因子-κB(TLRs-NF-κB)信号通路在儿童重症肺炎中的作用。【方法】回顾性分析2019年4〜12月本院收治的120例重症肺炎患儿的临床资料(观察组),选择同期健康体检儿童89例为对照组。采用酶联免疫吸附试验(ELISA)测定白细胞介素-6(IL-6)、IL-8、肿瘤坏死因子-α(TNF-α)、C反应蛋白(CRP)水平;采用流式细胞仪测定各组TLRs细胞限制存在型(TLR2、TLR4)阳性细胞百分比;采用Pearson相关性分析对TLR2、TLR4阳性率与IL-6、IL-8、TNF-α、CRP水平进行相关性分析。【结果】观察组患儿血清IL-6、IL-8、TNF-α、CRP水平均高于对照组,差异均有统计学意义(P<0.05)。观察组TLR2、TLR4阳性细胞百分比分别为(59.30±5.71)%、(59.70±9.48)%,对照组分别为(0.20±0.06)%、(0.40±09)%,差异均有统计学意义(P<0.05)。Pearson相关性分析结果显示,重症肺炎患儿TLRs-NF-κB信号通路中TLR4、TLR2阳性细胞百分比与炎性因子IL-6、IL-8、TNF-α、CRP水平均呈正相关性(P<0.05)。【结论】TLRs-NF-κB信号通路中的TLR4、TLR2阳性细胞在重症肺炎患儿中呈高表达,与炎症因子呈正相关性,以TLRs-NF-κB信号通路为靶点,能够指导临床诊疗。

【Objective】To investigate the role of Toll-like receptor-nuclear transcription factor-icB(TLRS-NF-κB)signaling pathway in children with severe pneumonia.【Methods】A total of 120 children with severe pneumonia from April 2019 to December 2019 were selected as observation group;A total of 89 healthy children were selected as the control group.The levels of interleukin-6(IL-6),interleukin-8(IL-8),tumor necrosis factor-α(TNF-α)and C-reactive protein(CRP)in children were determined by enzym-linked immunosor-bent assay(ELISA).The percentage of TLRS cells with restricted presence type(TLR2,TLR4)positive cells was determined by flow cytometry.The Pearson correlation analysis software was used to analyze the correla-tion between the positive rates of TLR2 and TLR4 and the levels of HMGB1,TNF-α and CRP.【Results】The levels of serum IL-6,IL-8,TNF-α and CRP in children with severe pneumonia in the observation group were higher than those in control group(P<0.05).The results of flow cytometry showed that the percentage of TLR2 positive cells in the observation group was(59.30±5.71)%,and the percentage of TLR4 positive cells in the observation group was(59.70±9.48)%,which were both higher than that in the control group(0.20±0.06)%,and the percentage of TLR4 positive cells was(0.40±09)%(P<0.05).Pearson correlation anlysis showed that TLR4 and TLR2 in TLRS-NF-κB signaling pathway were positively correlated with the levels of inflammatory factors IL-6,IL-8,TNF-α and CRP in children with severe pneumonia(P<0.05).【Conclusion】TLRS-NF-κB signaling pathway is highly expressed in children with severe pneumonia,which is strongly correlated with inflammatory factors.Strengthening TLRS-NF-κB signaling pathway determination can guide clinical diagnosis and treatment.