不同类型肠易激综合征患者血清及结肠黏膜IL-10、TNF-α水平与酪酪肽、密封蛋白-1的相关性分析

Correlation Analysis Between Serum and Colonic Mucosal IL-10,TNF-α,Tyrosin and Sealin-1 in Different Types of Irritable Bowel Syndrome

【目的】探讨不同类型肠易激综合征(IBS)患者血清及结肠黏膜白细胞介素-1O(IL-1O)、肿瘤坏死因子-α(TNF-α)水平与酪酪肽(PYY)、密封蛋白-1的相关性。【方法】90例IBS患者根据其类型分为腹泻组50例、便秘组40例,另选择同期的40例健康者作为对照组。比较三组患者血清和结肠黏膜中的IL-10、TNF-α、PYY、密封蛋白-1水平,并分析血清及结肠黏膜中IL-10、TNF-α水平与PYY、密封蛋白-1的相关性。【结果】对照组的IL-10、密封蛋白-1显著高于腹泻组,TNF-α、PYY水平显著低于腹泻组(P<0.05);腹泻组的IL-10、密封蛋白-1显著低于便秘组,TNF-α、PYY显著高于便秘组(P<0.05);对照组与便秘组的TNF-α比较差异无显著性(P>0.05)。三组结肠黏膜中IL-10的阳性表达率两两比较差异均有显著性(P<0.05);对照组结肠黏膜中TNF-α和PYY的阳性表达率显著低于腹泻组(P<0.05);三组密封蛋白-1的阳性表达率两两比较差异均有显著性(P<0.05);对照组结肠黏膜中TNF-α、PYY的阳性表达率与便秘组比较,差异均无显著性(P>0.05)。IBS血清及结肠黏膜IL-10与PYY无相关性(P>0.05),与密封蛋白-1呈正相关(P<0.05);IBS血清及结肠黏膜TNF-α与PYY呈正相关(P<0.05),与密封蛋白-1无相关性(P>0.05)。【结论】IBS患者存在抑炎因子与促炎因子失衡,临床可通过调节PYY与密封蛋白-1的表达,预防或控制IBS的发生、发展,进而降低患者的炎症反应,提高临床疗效。

【Objective】To study the correlation between serum and colonic mucosal IL-10,TNF-α,tyrosin(PYY)and sealin-1 in different types of irritable bowel syndrome(IBS).[Methods]The data of 90 patients with IBS,according to the symptomatic types,were divided into the diarrhea group(n=50)and the constipation group(n=40).In the same period,40 healthy people were selected as the control group.The levels of serum and colonic mucosa IL-10,TNF-α,tyrosin and sealin-1 were compared among the three groups.The correlation of IL-10,TNF-α,PYY and sealin-1 in colonic mucosal and serum was analyzed.【Results】Levels of IL-10 and sealin-1 in the control group were higher,while levels of TNF-α and PYY in the control group were lower than those in the diarrhea group(P<0.05).IL-10 and sealin-1 levels in the diarrhea group were lower than those in the constipation group;and TNF-α and PYY levels in the diarrhea,group were higher than those in the constipation group(P<0.05).There was no significant difference in TNF-α level between the control group and the constipation group(P>0.05).The positive expression rates of IL-10 in the colonic mucosa of the control group,diarrhea group and constipation group were 37.50%(15/40),24.00%(12/50),and 55.00%(22/40),respectively;The difference was statistically significant(P<0.05).The positive expression rate of TNF-α in the colonic mucosa of the control group was 22.50%(9/40),which was significantly lower than that in the diarrhea group by 68.00%(34/50)(P<0.05).the positive expression rate of PYY in the colonic mucosa of the control group was 27.50%(11/40),which was significantly lower than that in the diarrhea group(76.00%,38/50)(P<0.05).The positive expression rates of sealin-1 in the colonic mucosa of the control group,diarrhea group and constipation group were 32.50%(13/40),14.00%(7/50),75.00%(30/40),respectively;The difference between the three groups was statistically significant(P<0.05).The positive expression rate of TNF-α and PYY in the colonic mucosa of the control group was not statistically significant compared to the constipation group(P>0.05).There was no correlation between IL-10 and PYY in serum and colonic mucosa of IBS(P>0.05)»and the serum sealin-1 level was positively correlated with colonic mucosal sealin-1 level(P<0.05).TNF-α in serum and colonic mucosa of patients with IBS were posi-tively correlated with PYY(P<0.05),but not correlated with sealin-1(P〉0.05).【Conclusion】In patients with IBS,there is an imbalance between anti-inflammatory factors and pro-inflammatory factors.It can prevent or control the development of IBS by regulating the expression of PYY and sealin-1,thereby reducing the inflammatory response and promoting clinical efficacy.