摘要
【目的】初步明确槲皮素对短星翅蝗生长发育和主要解毒酶活性的影响,为防蝗生物农药的开发和利用提供依据。【方法】分别用0(CK)、0.001%、0.01%、0.1%和1%槲皮素溶液饲喂短星翅蝗3龄蝗蝻(雌虫),测定蝗蝻7 d存活率、干重、生长速率和活性氧簇(ROS)水平,并检测细胞色素P450(CYP450)、谷胱甘肽-S-转移酶(GST)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性。【结果】与对照组相比,0.01%、0.1%和1%槲皮素处理短星翅蝗3龄蝗蝻后,蝗蝻7 d存活率分别下降18.29%、37.69%和53.68%,干重分别下降23.74%、28.25%和30.25%,生长速率分别下降58.02%、69.02%和73.96%;蝗蝻7 d存活率、干重和生长速率与槲皮素浓度显著(P<0.05)线性负相关。ROS水平分别上升95.66%、173.56%和157.04%,CYP450活性分别上升118.09%、142.61%和174.87%,GST活性分别上升143.83%、194.41%和235.62%,SOD活性分别上升75.47%、97.37%和107.95%,CAT活性分别上升299.49%、282.23%和333.74%;ROS水平、CYP450活性和GST活性与槲皮素浓度显著(P<0.05)线性正相关;SOD活性和CAT活性与槲皮素浓度呈线性正相关关系,但相关性不显著(P>0.05)。【结论】槲皮素对短星翅蝗具有一定抑制生长作用,在短星翅蝗绿色防控和生物农药开发方面具有潜在应用价值。
【Objective】The effects of quercetin on the growth and development and main detoxification enzymes activities of Calliptamus abbreviatus were preliminarily determined,which provided the basis for the development and utilization of biological pesticides against Calliptamus abbreviatus.【Methods】The 3-year-old grasshoppers(female)were fed with 0(CK),0.001%,0.01%,0.1%and 1%quercetin solution,respectively.It was measured that the 7-day survival rate,dry weight,growth rate,reactive oxygen species(ROS)levels,and the activities of cytochrome P450(CYP450),glutathione S-transferase(GST),superoxide dismutase(SOD)and catalase(CAT).【Results】Compared with CK,after treated with 0.01%,0.1%and 1%quercetin,the 7-day survival rate decreased by 18.29%,37.69%and 53.68%,dry weight decreased by 23.74%,28.25%and 30.25%,and growth rate decreased by 58.02%,69.02%and 73.96%,respectively.The 7-day survival rate,dry weight and growth rate were significantly negatively correlated with quercetin concentration(P<0.05).ROS levels increased by 95.66%,173.56%and 157.04%,the activities of CYP450 increased by 118.09%,142.61%and 174.87%,the activities of GST increased by 143.83%,194.41%and 235.62%,the activities of SOD increased by 75.47%,97.37%and 107.95%,the activities of CAT increased by 299.49%,282.23% and 333.74%. ROS levels,the activities of CYP450 and the activities of GST were significantly positively correlated with quercetin concentration(P<0.05). The activities of SOD and the activities of CAT were showing positively correlated with quercetin concentration,but the correlation had no significant difference(P>0.05).【Conclusion】Quercetin has a certain inhibitory effect on the growth of Calliptamus abbreviatus,and has potential application value in the biological pesticide for the green control of Calliptamus abbreviates.
作者
黄训兵
王馨超
李辉
HUANG Xunbing;WANG Xinchao;LI Hui(College of Agriculture and Forestry Science,Linyi University,Linyi 276000,China)
出处
《北方农业学报》
2021年第2期71-77,共7页
Journal of Northern Agriculture
基金
山东省农业科技资金(林业科技创新)项目(2019LY005)
临沂大学大学生创新训练项目(X202010452027)
山东省自然科学基金博士基金项目(ZR2019BC030)。
关键词
槲皮素
短星翅蝗
存活率
生长速率
酶活性
Quercetin
Calliptamus abbreviatus
Survival rate
Growth rate
Enzymatic activity