加工番茄幼苗对盐胁迫的响应

Response of processed tomato seedlings to salt stress

【目的】研究盐胁迫对加工番茄幼苗的影响,明确加工番茄正常生长的最大盐浓度,为加工番茄栽培生产和耐盐育种提供参考。【方法】以加工番茄里格尔87-5幼苗为试验材料,在苗期分别浇灌0(CK)、50、100、150、200 mmol/L NaCl溶液,分析加工番茄形态、生理指标及K^(+)和Na^(+)在不同浓度NaCl溶液下的变化。【结果】随着NaCl浓度的升高,加工番茄幼苗的株高和茎粗表现降低趋势。在NaCl胁迫下,加工番茄叶片中SOD活性、POD活性、CAT活性、MDA含量、Pro含量显著增加(P<0.05)。不同浓度NaCl胁迫下,各器官中K^(+)含量为茎>叶>根;各器官中Na^(+)含量在NaCl浓度小于150 mmol/L时为根>茎>叶,在NaCl浓度大于150 mmol/L时为茎>根>叶。【结论】随着NaCl浓度的增加,加工番茄幼苗的生长受到抑制,抗氧化酶含量随NaCl浓度的增加而增加;NaCl浓度大于150 mmol/L时,细胞膜系统受损,离子平衡被打破,影响了植株对离子的选择性吸收,植株不能正常生长。

【Objective】To study the effect of salt stress on processed tomato seedlings,to clarify the maximum salt concentration for normal growth of processed tomato,and to provide a reference for processed tomato cultivation and production and salt-tolerant breeding.【Methods】The seedlings of processed tomato Riegel 87-5 was used as test materials,and 0(CK),50,100,150,200 mmol/L NaCl solution was irrigated at the seedling stage,to analyze the morphology,physiological indexes and the change of K^(+)and Na^(+)under different concentrations of NaCl solution of processed tomato.【Results】With the increase of NaCl concentration,the plant height and stem thickness of processed tomato seedlings decreased.Under NaCl stress,SOD activity,POD activity,CAT activity,MDA content,and Pro content in processed tomato leaves increased significantly(P<0.05).Under different concentrations of NaCl stress,the K^(+)content in each organ was stem>leaf>root;when the NaCl concentration was less than 150 mmol/L,the Na^(+)content in each organ was root>stem>leaf,and when the NaCl concentration was greater than 150 mmol/L,it was the stem>root>leaf.【Conclusion】With the increase of NaCl concentration,the growth of processed tomato seedlings was inhibited,and the content of antioxidant enzyme increased with the increase of NaCl concentration;when the NaCl concentration was greater than 150 mmol/L,the cell membrane was damaged,the ion balance was broken,which affected the selective absorption of ions by the plant,and the plant cannot grow normally.