枣裂果相关的基因筛选

Screening of Fruit Cracking Genes in the Process of Fruit Cracking of Jujube

为了解枣果实开裂与基因表达的关系,并筛选出与裂果相关的基因,本研究以易裂品种‘伏脆蜜’为材料,利用Illumina测序平台对裂果和非裂果部位果皮进行转录组测序分析,结果表明裂果部位和非裂果部位两个样品中分别获得10025和10041个表达基因,其中9802个基因在两个样品中都有表达,有223个基因在裂果部位中特异表达,有239个基因在非裂果部位中特异表达;在裂果果皮部位对比非裂果果皮部位中筛选出差异表达基因161个,其中上调表达基因39个,下调表达基因122个。通过对基因功能和KEGG代谢通路上基因差异表达筛选,主要在合成木质素的通路上发现2个基因(gene16874和gene17086);在植物激素信号转导中,发现1个AUX/IAA(gene26281)、1个ARF(gene22744)和1个SAUR(gene7576)及1个JAZ(gene2607)基因;在植物病原体相互作用代谢通路中,2个CML(gene19427和gene19466)基因;在淀粉和蔗糖代谢通路中,有1个β-淀粉酶(gene32293)基因和1个Endoglucanase 12(gene3673)基因。结合转录组数据分析和利用RT-qPCR验证,上述10个差异表达基因在裂果果皮中都高表达,说明这10个基因可能参与裂果的发生过程。

In order to understand the relationship between fruit dehiscence and gene expression of jujube,and to screen out the genes related to fruit dehiscence.The fissure-prone variety’Fucuimi’was used as the material,and we used Illumina sequencing platform,to perform transcriptome sequencing was performed on rind of cracked and non-cracked fruit.The transcriptome sequencing analysis showed that there were 10025 and 10041 expressed genes in the cracked and non-cracked fruit.Among them,9802 genes were expressed in both samples,223 genes were specifically expressed in the cracked fruit,and 239 genes were specifically expressed in the non-cracked fruit.161 differentially expressed genes were screened from cracked parts vs non-cracked parts.Among which 39 genes were up-regulated and 122 were down-regulated.By screening for gene function and KEGG metabolic pathways,gene16874 and gene17086 genes were differentially expressed in the pathway of lignin synthesis.One AUX/IAA(gene26281),one ARF(gene22744),one SAUR(gene7576)and one JAZ(gene2607)genes were differentially expressed in plant hormone signal transduction.Two CML(gene19427 and gene19466)were differentially expressed in the metabolic pathways of plant pathogen interaction.In the starch and sucrose metabolic pathways,the gene of beta-amylase(gene32293)was differentially expressed.Differential expression of genes regulating Endoglucanase 12(gene3673).All the 10 differentially expressed genes were highly expressed in the peel of cracked fruit.Through RT-PCR,it was found that 10 genes were also highly expressed in the peel of cracked fruit,indicating that these 10 genes may affect the structure of the peel and the development of the peel,and participate in the occurrence of cracked fruit.