扁蓿豆DNA甲基转移酶基因MrMET1的克隆及表达分析

Cloning and Expression Analysis of DNA Methyltransferase Gene MrMET1 in Medicago ruthenica

DNA甲基化是一类重要的表观遗传修饰,在植物对非生物胁迫的响应中发挥重要作用。DNA的甲基化需要DNA甲基转移酶催化。为挖掘扁蓿豆中DNA甲基转移酶基因,解析其是否参与扁蓿豆抗逆性应答,本研究利用RT-PCR技术从扁蓿豆中克隆到1个胞嘧啶5-甲基DNA转移酶基因,该基因含1071 bp的开放阅读框,编码357个氨基酸。生物信息学分析确定该基因为DNA甲基转移酶1 MrMET1基因。实时荧光定量PCR显示,MrMET1基因在不同非生物胁迫(低温,干旱,NaCl)及ABA处理下,不同时间表达量呈现不同的变化趋势,推测该基因参与了扁蓿豆对非生物胁迫的响应。本研究结果对深入解析METs基因的功能,理解牧草对非生物逆境胁迫响应机制具有一定的参考意义。

DNA methylation is an epigenetic modification,which plays an important role in plant response to abiotic stress.DNA methylation is catalyzed by DNA methyltransferases.To explore the DNA methyltransferase gene and to clarify whether it is involved in the resistance response of Medicago ruthenica,one cytosine-5 DNA methyltransferase was cloned using the RT-PCR.It had an open reading frame of 1071 bp,encoding 357 amino acids.Bioinformatics analysis confirmed that the gene was a cytosine DNA methyltransferase 1 MrMET1 gene.qRT-PCR analysis showed that MrMET1 gene had differential expressions at different stages under the abiotic stress(cold,dehydrin,Na Cl)and ABA treatment,it suggested that the gene might be participated in the response to the abiotic stress.So these results are useful for further in depth studying the function of METs and understanding the mechanism of the forage in response to abiotic stress.