基于土大黄苷定性定量检测的大黄类中药掺伪鉴定方法研究进展

Research progress on identification of Rhubarb based on rhapontin detection

大黄为临床常用中药,且处方众多,《中国药典》2015年版中共收载了153个含大黄的中药制剂。近年来,由于多种因素影响,大黄及其制剂中出现较多掺伪现象,因此,加强大黄真伪鉴定对保障临床用药安全有效具有重要意义。土大黄苷为大黄真伪鉴定指标化成分,在《中国药典》2015年版中用于大黄饮片、九味肝泰胶囊、三黄片和致康胶囊的定性鉴定,但相对于153个含大黄的制剂总量而言,其应用略显不足。另一方面而言,更多大黄制剂的检测仍有待完善。由于大黄在各制剂中的用量和制剂种类的差异,土大黄苷的检测方法也不尽相同。因此,笔者结合近20年国内外文献,对土大黄苷的定性定量检测方法与条件进行总结,为进一步推广土大黄苷在含大黄制剂真伪鉴定中的鉴定作用提供方法参考。

Rhubarb is one of the most widely used traditional Chinese medicines.As recorded in Pharmacopoeia of the People′s Republic of China(2015th edtion),there are about 153 kinds of Chinese traditional compound medicine which contain rhubarb.However,the falsifies of rhubarb influence clinical application seriously.Rhapontin,an important identification marker,is used for the identification of crude drug of rhubarb,Jiuweigantai Capsules,Sanhuang Tablets and Chiho Capsules as recorded in Pharmacopoeia of the People′s Republic of China.However,it is still insufficient for so many agents.What′s more,the detection methods vary from the different amount of rhubarb and different preparations.In this paper,several detection methods of rhapontin were summarized based on domestic and foreign literatures in the past 20 years,which provided valuable references for further researches on the identification of rhubarb.