复方鱼腥草合剂体外抗流感病毒作用研究

Effect of compound houttuynia mixture on influenza virus in vitro

复方鱼腥草合剂属于OTC甲类药物,成分为鱼腥草、黄芩、板蓝根、连翘和金银花五味药材。鱼腥草合剂作为一种中药复方制剂,药理作用广泛,具有清热解毒作用,临床用于治疗外感风热引起的咽喉疼痛、急性咽炎和扁桃腺炎。本研究对复方鱼腥草合剂体外抗流感病毒作用及初步作用机制进行了探索。采用细胞病变效应(cytopathic effects,CPE)、Western blot、实时荧光定量PCR法(quantitive reverse transcription PCR,qRT-PCR)和病毒滴度等多种方法测定了复方鱼腥草合剂的抗流感病毒作用。采用时间进程分析研究鱼腥草合剂作用于流感病毒复制周期中的阶段。结果表明,复方鱼腥草合剂体外具有广谱抗流感病毒作用,对流感病毒国际通用株、我国临床分离株耐药株、高致病性禽流感病毒H5N1和H7N9均有抑制作用,主要作用于流感病毒复制的早期阶段。

Compound houttuynia mixture belongs to OTC class A medicine,which is made from Houttuynia cordata,Scutellaria baicalensis,Radix Isatidis,Forsythia,and Lonicera.As a kind of compound preparation of traditional Chinese medicine,houttuynia cordata mixture has extensive pharmacological effects,for example,clearing away heat and detoxifying,thus it is used for the sore throat,acute pharyngitis,and tonsillitis with wind-heat syndrome.In this study,the antiviral activity against influenza viruses and the primary mechanism of compound houttuynia mixture was evaluated.The antiviral effect of compound houttuynia mixture was determined by cytopathic effects(CPE),Western blot,quantitive reverse transcription PCR(qRT-PCR),and virus titer assays.The effect of houttuynia mixture on the replication cycle of influenza virus was evaluated by time-of-addition assay.In conclusion,the results showed that the compound houttuynia mixture had a broad-spectrum effect against influenza virus,including the international common influenza virus strains,the drug-resistant strains and the highly pathogenic avian influenza viruses H5N1 and H7N9.It mainly impairs the early stage of the viral replication.