药用植物大麻C2H2基因家族鉴定与表达分析

Identification and expression analysis of the C2H2 gene family in Cannabis sativa L.

C2H2型转录因子在植物生长发育及次生代谢调控中发挥重要作用,本文利用生物信息学从基因组层面鉴定大麻(Cannabis sativa L.)C2H2基因家族成员,基于大麻基因组数据及转录组数据,使用TBtools、MEGA软件以及NCBI、PlantTFDB、ExPASy、HMMSCAN、MEME、WoLFPSORT、PlantCARE等在线网站对其基因信息、染色体定位、进化关系、保守基序、共线性关系等进行分析。结果显示,大麻中含有30个C2H2型基因家族成员(CsC2H2-1~CsC2H2-30),分布在大麻的9条染色体上,氨基酸大小介于138~635个氨基酸,理论等电点介于5.85~9.52,蛋白质分子质量介于15909.48~68445.53 Da。转录组结果分析显示,CsC2H2在大麻Diku品种的雌花、苞片、叶、茎及不同品种雌花中表达量均存在差异。实时荧光定量PCR结果验证了CsC2H2-1、CsC2H2-5、CsC2H2-19在Diku品种的雌花及苞片中表达显著,为深入研究C2H2基因家族功能及大麻优质品种的选育提供理论参考。

C2 H2 transcription factors play an important role in plant growth,development and the regulation of secondary metabolism.This article identifies members of the C2 H2 gene family in Cannabis sativa L.at the genome level.Chromosomal location and linkage,evolutionary relationships,and identification of conserved motifs was determined from the C.sativa genome and transcriptome data using bioinformatics tools and on-line websites such as TBtools,MEGA software,NCBI,PlantTFDB,ExPASy,HMMSCAN,MEME,WoLFPSORT and PlantCARE.The results show that C.sativa contains 30 members of the C2 H2 gene family(named CsC2 H2-1-CsC2 H2-30)distributed on 9 chromosomes.The encoded proteins range in length from 138 to 635 amino acids,and the theoretical isoelectric points range from 5.85 to 9.52.Molecular weights range from 15909.48 to 68445.53 Da.Transcriptome analysis showed that CsC2 H2 was differentially expressed in the female flowers,bracts,leaves,and stems of the Diku variety and female flowers of nine different varieties of C.sativa.Quantitative real-time PCR verified that CsC2 H2-1,CsC2 H2-5,and CsC2 H2-19 were significantly expressed in the female flowers and bracts of the Diku variety.This provides a theoretical basis for in-depth study of the function of the C2 H2 gene family and the breeding of high-quality C.sativa varieties.