外周血白细胞形态学参数在甲型、乙型流行性感冒诊断中的应用

Application of leukocyte morphological parameters in the diagnosis of influenza A and B

目的探讨甲型、乙型流行性感冒(简称流感)患者外周血常规检测中白细胞形态学参数(LMP)的变化特点及其在临床诊断中的应用。方法选取2019年7月—2020年1月上海交通大学医学院附属瑞金医院甲型流感患者77例(甲型流感组)和乙型流感患者109例(乙型流感组),另选取同期101名健康体检者作为健康对照组。检测所有研究对象血常规相关指标、C反应蛋白(CRP)和甲型流感、乙型流感病毒。结果甲型流感组CRP阳性患者28例,阴性患者49例;乙型流感组CRP阳性患者23例,阴性患者85例。健康对照组CRP和甲型流感、乙型流感病毒检测均为阴性。3个组CRP阴性患者中,中性粒细胞百分比(NEUT%)、单核细胞百分比(MO%)、单核细胞前向散射光中心位置(Mon_FSC_CenterPos)、单核细胞前向散射光分布宽度(Mon_FSC_Width)、高荧光强度细胞比例(HFC%)差异均有统计学意义(P<0.01)。甲型流感组和乙型流感组CRP阳性患者中,HFC%、Mon_FSC_CenterPos差异均有统计学意义(P<0.05)。结论Mon_FSC_CenterPos具有较好的甲型流感、乙型流感鉴别诊断能力,可以作为一个良好的生物学指标。

Objective To investigate the characteristics of leukocyte morphological parameters(LMP)in diagnosing influenza A and B.Methods A total of 77 patients with influenza A,109 patients with influenza B and 101 healthy subjects were enrolled in Shanghai Jiao Tong University School of Medicine Ruijin Hospital from July 2019 to January 2020 and were determined for blood routine test,C-reactive protein(CRP),influenza A virus and influenza B virus.Results There were 28 CRP-positive patients and 49 CRP-negative patients in influenza A group.There were 23 CRP-positive patients and 85 CRP-negative patients in influenza B group.CRP and influenza A and B viruses were negative in healthy control group.In patients with influenza A and influenza B and healthy subjects with negative CRP results,the percentage of neutrophil(NEUT%),the percentage of monocyte(MO%),monocyte forward scattered light central position(Mon_FSC_CenterPos),monocyte forward scattered light distribution width(Mon_FSC_Width)and high fluorescence intensity cell percentage(HFC%)had statistical significance among the 3 groups(P<0.01).Among CRP-positive patients with influenza A and B,HFC%and Mon_FSC_CenterPos had statistical significance in influenza A and B groups(P<0.05).Conclusions Mon_FSC_CenterPos can be used as a good biomarker in differential diagnosis of influenza A and B.