肺癌患者肺组织微生物多样性及丰度变化研究

Study of microbial diversity and abundance in lung tissue of patients with lung cancer

目的分析肺癌患者不同形态肺部微生物组学特征。方法收集2019年该院收治的19例肺癌行肺癌切除术患者的肺组织(分别为距癌变1 cm处的组织及相对癌变部位远离的组织),进一步分离标本,DNA提取及通过Illumina平台采用16S rDNA高通量测序的方法,检测肺癌患者肺组织的微生物组学特征;并使用Metastats两两比较和Lefse判别分析等统计学方法比较不同组别的微生物多样性和差异性。结果肺癌患者肺部微生物多样性分析结果显示,属水平主要包括苍白杆菌属(19.4%)、沉积物杆状菌属(11.3%)、不动杆菌属(6.6%)等;A1组(距离癌变1 cm处的组织)和B1组(远离癌变部位的组织)在分类群(Top>20)中主要包括苍白杆菌属、沉积物杆状菌属、不动细菌属、贪铜菌属、拟无枝酸菌等,两组差异无统计学意义(P>0.05);A2组(小细胞癌肺组织)和B2组(非小细胞癌肺组织)物种多样性分析两组差异无统计学意义(P>0.05),物种差异性分析显示,A2组中物种丰度相对较高的为叶杆菌属,而在B2组内丰度相对较高的物种分别为假单胞菌、嗜热油菌纲、绿弯菌门、绿弯菌目、绿弯菌科、弧菌目、假交替单胞菌科、假交替单胞菌属、绿线菌属。结论肺癌患者肺组织不同部位的微生物多样性并无明显差异,小细胞癌和非小细胞癌微生物的多样性并无明显差异,但非小细胞癌肺部存在更多的优势菌落。

Objective To analyze the characteristics of lung microbiome with different morphologies in the patients with lung cancer.Methods The lung tissues(1cm from canceration and relatively distant from the cancerous site)from 19 inpatients with lung cancer treated by lung cancer resection in this hospital during 2019 were collected,and further isolated and conducted the DNA extraction.The 16S rDNA high-throughput sequencing method through the Illumina platform was adopted to detect the microbiome characteristics of lung tissues in the patients with lung cancer.Moreover,the statistical method of Metastats pairwise comparison and Lefse discriminant analysis were used to compare the microbial diversity and difference among different groups.Results The lung microbial diversity analysis results of the lung cancer patients showed that the genus level mainly included pallidobacterium(19.4%),bacilli of sediment(11.3%),Acinetobacter(6.6%),etc.;the group A1(tissue at 1cm from canceration)and group B1(tissues distant from the cancerous site)mainly included Ochrobactrum,sedinibacterium,Acinetobacter,cupriavidus and Amycolatopsis in the taxonomic groups(Top>20),there was no statistically significant difference between the two groups(P>0.05).The species diversity analysis showed no statistical difference between the A2 group(small cancer lung tissue)and the B2 group(non-small cell lung cancer tissue)(P>0.05).The species diversity analysis showed that the species abundance of phyllobacterium in the A2 group was high,while the species with relatively high abundance in the B2 group were Pseudomonas and thermooleophilia,chloroplexi,chloroflexaceae,vibrionales,pseudoalteronaceae,Pseudoalteromonas and chloronema.Conclusion There is no difference in microbial diversity in different parts of lung tissues of the patients with lung cancer,and there is no difference between small cell cancer and non-small cell cancer.The more superiority bacterial colonies exist in the lung of small cell cancer.