羊BMPR-IB基因非同义单核苷酸多态性的生物信息学分析

Bioinformatics Analysis of Non-Synonymous Single Nucleotide Polymorphisms of Sheep BMPR-IB Gene

以羊BMPR-IB基因为研究对象,采用生物信息学方法,对该基因潜在的、具有生物学功能的非同义单核苷酸多态性位点(nsSNPs)进行预测,通过SIFI、PolyPhen-2、PROVEN等软件分析预测nsSNPs是否对BMPR-IB蛋白的结构及功能产生影响;通过I-Mutant 3.0、BDM-PUB、GPS-SUMO、Consurf等软件分析预测有害位点nsSNPs对蛋白质稳定性、泛素化修饰位点、磷酸化修饰位点、氨基酸进化位保守水平等是否有影响。结果表明:BMPR-IB基因存在9个nsSNPs位点,其中Q305R、R469K两个位点为主要的有害突变;9个nsSNPs位点BMPR-IB蛋白稳定性均有所降低,其中C35Y、C45S、R44H的稳定性大大降低。预测结果显示,Q305R、R469K为羊BMPR-IB基因的潜在功能性位点。

Taking the sheep BMPR-IB gene as the research object,bioinformatics methods were used to predict the potential and biological non-synonymous single nucleotide polymorphisms(nsSNPs)of the gene. It was analyzed and predicted whether nsSNPs would affect the structure and function of BMPR-IB protein by using SIFI,PolyPhen-2,PROVEN and other software.It was also analyzed and predicted whether nsSNPs harmful sites would affect protein stability,ubiquitination modification sites,phosphorylation modification sites,and amino acid evolutionary conservation levels by using software such as I-Mutant 3.0,BDM-PUB,GPS-SUMO,and Consurf.The results showed that 9 nsSNPs sites were screened out,of which 2 sites Q305R and R469K were the main harmful mutations;the stability of BMPR-IB protein at 9 nsSNPs sites was reduced,of which the stability of C35 Y,C45S,and R44H was greatly reduced.In conclusion,that the potential functional sites of sheep BMPR-IB gene were Q305R and R469K.