RNF2在结直肠癌中的表达及其对结直肠癌细胞生物学行为的影响

Expression of RNF2 in colorectal cancer tissues and its effect on biological behavior of colorectal cancer cells

目的探讨环指蛋白2 (RNF2)在结直肠癌中的表达及其对结直肠癌细胞生物学行为的影响。方法采用免疫组织化学法检测RNF2在结直肠癌组织和癌旁正常组织中的表达,并利用过表达的慢病毒转染HCT116细胞构建过表达RNF2的HCT116细胞系,通过CCK-8法、划痕实验、Transwell实验检测过表达RNF2对结直肠癌细胞增殖、迁移、侵袭等生物学行为的影响。结果结直肠癌组织中RNF2阳性表达率高于癌旁正常组织(P <0.05);不同年龄、组织学类型、分化程度、Dukes分期、淋巴结转移的结直肠癌患者的RNF2表达阳性率比较,差异有统计学意义(P <0.05);HCT116组、HCT116-control组、HCT116-RNF2组HCT116细胞增殖、迁移能力及侵袭能力比较,差异有统计学意义(P <0.05);HCT116-RNF2组细胞增殖速度、迁移能力及侵袭能力均高于HCT116-control组和HCT116组(P <0.05)。结论 RNF2在结直肠癌中呈高表达,且过表达RNF2可促进结直肠癌细胞迁移、增殖、侵袭能力,可为结直肠癌治疗提供新的治疗靶点。

Objective To investigate Expression of ring finger protein 2(RNF2) in colorectal cancer(CRC)tissues and its relationship with biological characteristics. Methods The expression of RNF2 in CRC tissues and adjacent normal tissues was detected by immunohistochemistry. RNF2-overexpressing HCT-116 colorectal cancer cell lines were constructed, then the effects of overexpression of RNF2 on proliferation, migration, and invasion of colorectal cancer cells were studied by CCK-8, scratch test and Transwell chamber. Results The positive expression rate of RNF2 in CRC tissues was higher than that in adjacent normal tissues(P < 0.05). The expression of RNF2 showed significant difference in CRC patients with different age, histological types, differentiation degree,Dukes stage, and lymph node metastasis(P < 0.05). HCT-116 cell proliferation rate, migration ability and invasive ability in HCT-116 group, HCT-116-control group, and HCT-116-RNF2 group respectively showed statistically significant differences(P < 0.05). The proliferation rate, migration ability, and invasion ability of HCT-116-RNF2 cells were higher than those of HCT-116-control and HCT-116(P <0.05). Conclusion RNF2 is highly expressed in CRC, and the overexpression of RNF2 can promote the migration, proliferation, and invasion of CRC cells, which can be a new therapeutic target for CRC treatment.