摘要
对液态奶中STC残留的两种快速检测方法进行了优化和改进。对UV-VIS方法进行单因素筛选和正交设计优化的结果表明,该方法的检测波长为451 nm,检出限为2.699 mg/L,回收率在93.63%~95.47%之间,RSD为2.21%。目视比色法的操作流程与UV-VIS相比,操作更加简便,时间更短,在一定量牛奶中加入4种常规试剂,待显色反应5 min后直接用溶液颜色与标准比色卡对照判读。若样品溶液颜色比10 mg/L的标准液颜色深,即表明样品中的STC超过规定含量。两种检测方法均具有准确度高、成本低廉,快速便捷的优势,适用于液态奶生产企业和奶牛场现场快速检测。
In this paper,two rapid detection methods for STC residue in liquid milk were optimized and improved.Optimization of UV-VIS method for the detection of STC in milk by single factor and orthogonal design methods showed that selected detection wave⁃length was 451 nm,the limit of detection was 2.699 mg/L,the recovery was ranged between 93.63%-95.47%,and the RSD was 2.21%.Compared with UV-VIS,the operation procedure of visual colorimetry only different from that of crystal violet indicator was added before the chromogenic agent was added.After 5 min of reaction,color of the sample solution was compared with the standard colorimetric card.The sample solution was darker than that of the 10 mg/L solution of standard colorimetric card it indicates that the STC in the sample ex⁃ceeds the specified concentration.Both methods was has the advantages of high accuracy,low cost,fast and convenient,and were suitable for rapid on-site detection in liquid milk production enterprises and dairy farms.
作者
姚明奇
斯琴朝克图
吴菘建
戴余军
姜益泉
张杨
章爱群
YAO Mingqi;HARNUD Sechenchogt;WU Songjian;DAI Yujun;JIANG Yiquan;ZHANG Yang;ZHANG Aiqun(College of Plant Science and Technology of Hubei Engineering University,Xiaogan 432000,China;Hubei Province Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients,Xiaogan 432000,China;Research Center for Ecotoxicology and food Safety of Hubei Engineering Univesity,Xiaogan 432000,China)
出处
《中国乳品工业》
CAS
北大核心
2021年第5期48-53,64,共7页
China Dairy Industry
基金
湖北省教育厅优秀创新团队项目(T201716)
湖北工程学院科学研究项目(201609)。