穿心莲内酯抗心律失常作用及机制研究

Anti-arrhythmic Effects and Mechanism of Andrographolide

目的:研究穿心莲内酯对大鼠心律失常的作用机制。方法:将2019年10月-2020年10月从江西省实验动物中心购买的90只清洁级雄性大鼠,随机分为氯仿诱发心律失常组(A组,n=10)和氯仿诱发心律失常并穿心莲内酯组(B组,n=10);肾上腺素诱发心律失常组(C组,n=10)和肾上腺素诱发心律失常并穿心莲内酯组(D组,n=10);氯化钙诱发心律失常组(E组,n=10)和氯化钙诱发心律失常并穿心莲内酯组(F组,n=10);乌头碱诱发心律失常组(G组,n=10)和乌头碱诱发心律失常并穿心莲内酯组(H组,n=10);正常对照组(I组,n=10)。观察A~F组心律失常出现时间和心律失常持续时间,比较G、H组室性早搏(VP)、室性心动过速(VT)和室颤(VF)的持续时间,比较A~I组大鼠血清Ca2+、LDH水平。结果:B组与A组、D组与C组、F组与E组比较,心律失常出现时间均显著推迟,心律失常持续时间均显著缩短,差异均有统计学意义(P<0.05)。H组VP、VT、VF均显著短于G组,差异均有统计学意义(P<0.05)。与I组比较,A、C、E、G组大鼠Ca2+、LDH水平均显著升高(P<0.05);B组与A组、D组与C组、F组与E组、H组与G组比较,血清中Ca2+、LDH水平均显著降低(P<0.05)。结论:穿心莲内酯对氯仿、肾上腺素、氯化钙、乌头碱引起心律失常具有对抗作用,能够降低血清LDH与Ca2+水平,是抗心律失常的有效药物。

Objective:To study the mechanism of Andrographolide on arrhythmia in rats.Method:A total of 90 clean male rats who purchased from Jiangxi Provincial Laboratory Animal Center from October 2019 to October 2020 were randomly divided into Chloroform-induced arrhythmia group(group A,n=10)and Chloroform-induced arrhythmia combined with Andrographolide group(group B,n=10),Adrenerinol-induced arrhythmias(group C,n=10)and Adrenerinol-induced arrhythmias combined with Andrographolide group(group D,n=10),CaCl2-induced arrhythmia group(group E,n=10)and CaCl2-induced arrhythmia combined with Andrographolide group(F group,n=10),Aconitonine-induced arrhythmia group(group G,n=10)and Aconitine-induced arrhythmia combined with Andrographolide group(group H,n=10),normal control group(group I,n=10).The occurrence time and duration of arrhythmia in group A to F were observed.The duration of ventricular premature beats(VP),ventricular tachycardia(VT)and ventricular fibrillation(VF)in group G and group H were compared.The levels of serum Ca2+and LDH in group A to I were compared.Result:Compared with group A,group B,group D and group C,and group F and group E,the occurrence time of arrhythmia were significantly delayed and the duration of arrhythmia were significantly shortened,the differences were statistically significant(P<0.05).VP,VT and VF in group H were significantly shorter than those in group G,the differences were statistically significant(P<0.05).Compared with group I,the Ca2+and LDH levels in group A,group C,group E and group G were significantly increased(P<0.05).Compared with group A,group D,group C,group F and group E,and group H and group G,serum Ca2+and LDH levels were significantly decreased(P<0.05).Conclusion:Andrographolide can antagonize arrhythmia induced by chloroform,Adrenerinol,calcium chloride and aconitine,reduce serum LDH and Ca2+levels,and is an effective drug against arrhythmia.