HPV感染宫颈癌组织miR-222-3p的表达及对宫颈癌细胞凋亡的影响

Expression of miR-222-3p in cervical cancer infected with HPV and its effect on the apoptosis of cervical cancer cells

目的观察微小RNA(miR-222-3p)在高危型人乳头瘤病毒(HPV)感染宫颈癌组织中的表达情况,探讨其对HPV感染的宫颈癌细胞凋亡和同源结构域相互作用蛋白激酶2(HIPK2)通路的影响。方法:收集本院HPV阳性宫颈癌组织标本、HPV阴性宫颈癌组织标本及正常宫颈组织标本各10份,采用实时荧光定量聚合酶链反应(qT-PCR)及免疫组化法检测标本中miR-222-3p及HIPK2表达水平。取HPV感染的Siha细胞随机分为对照组、miR-222-3p低表达(Si-miR-222-3p)组、miR-222-3p低表达阴性对照(si-NC)组、HIPK2高表达组(Ad-HIPK2)组、HIPK2高表达阴性对照(Ad-NC)组。各组转染后,用qT-PCR法检测miR-222-3p及HIPK2 mRNA表达水平;噻唑蓝法检测细胞增殖率,流式细胞仪分析细胞凋亡率;Western blot检测HIPK2及其下游相关凋亡蛋白(capase-3)、P53、P57、促凋亡蛋白Bax的表达。将Si-miR-222-3p与HIPK2低表达腺病毒(si-pcD-HIPK2)共转染细胞,分为Si-miR-222-3p+si-pcD-HIPK2组、si-NC+si-pcD-HIPK2组、Si-miR-222-3p+si-pcD-NC组、si-NC+si-pcD-NC组及对照组,检测各组细胞miR-222-3p、HIPK2 mRNA表达及凋亡率变化。结果与正常宫颈组织(1.08±0.09、1.01±0.08)相比,HPV阳性及阴性宫颈癌组织标本miR-222-3p(1.62±0.15及2.26±0.25)及HIPK2(2.42±0.26及1.66±0.24)表达均升高(P<0.05);与HPV阴性宫颈癌组织相比,HPV阳性宫颈癌组织miR-222-3p表达升高(P<0.05),HIPK2表达降低(P<0.05)。与对照组(10.01±0.60、1.61±0.16、1.09±0.12、1.07±0.09、1.06±0.10、1.05±0.09、100.00±0.00、2.28±0.21)比较,si-miR-222-3p组及Ad-HIPK2组细胞凋亡率(31.29±3.35及32.33±3.24)及HIPK(2.62±0.25及2.76±0.27)、capase-3(1.83±0.18及1.86±0.18)、P53(1.75±0.17及1.79±0.16)、P57(1.80±0.15及1.94±0.19)、Bax(1.96±0.19及1.94±0.18)蛋白表达升高(P<0.05),增殖率(71.42±7.15及72.46±7.26)降低(P<0.05);si-miR-222-3p组miR-222-3p(1.42±0.15)表达降低(P<0.05),Ad-HIPK2组miR-222-3p(2.46±0.26)表达无显著变化(P<0.05);Si-NC组及Ad-NC组上述指标与对照组相比差异无统计学意义(均P>0.05)。与对照组(10.09±1.10)比较,Si-NC+si-pcD-HIPK2组细胞凋亡率(2.21±0.40)降低(P<0.05),si-miR-222-3p+si-pcD-NC组细胞凋亡率(32.13±3.28)升高(P<0.05);与si-miR-222-3p+si-pcD-NC组(32.13±3.28)相比,si-miR-222-3p+si-pcD-HIPK2组细胞凋亡率(10.19±1.29)降低(P<0.05);Si-NC+si-pcD-NC组(10.03±1.16)与对照组(10.09±1.10)相比,上述指标差异无统计学意义(P>0.05)。结论miR-222-3p在HPV阳性宫颈癌组织中表达异常升高,抑制miR-222-3p表达可通过靶向上调HIPK2表达促进HPV感染的宫颈癌细胞凋亡。

Objective To observe the expression of microRNA(miR-222-3 p)in cervical cancer tissue infected with high-risk human papillomavirus(HPV)and to explore its effects on apoptosis and the homeodomain interacting protein kinase 2(HIPK2)pathway in cervical cancer cells infected with HPV.Methods Ten specimens of HPV-positive cervical cancer,10 specimens of HPV-negative cervical cancer,and 10 specimens of normal cervical tissue were collected.The levels of miR-222-3 p and HIPK2 expression were determined using real-time fluorescence quantitative PCR(qT-PCR)and immunohistochemistry.SiHa cells infected with HPV were randomly divided into a control group,a group with low expression of miR-222-3 p(si-miR-222-3 p),a negative control group with low expression of miR-222-3 p(si-NC),a group with high expression of HIPK2(Ad-HIPK2)group,and a negative control group with high expression of HIPK2(Ad-NC).After transfection,the levels of miR-222-3 p and HIPK2 mRNA expression were determined using qT-PCR.The rate of cell proliferation was determined using the thiazolyl blue method and the rate of apoptosis was determined using flow cytometry.The expression of HIPK2 and its related downstream pro-apoptotic protein(capase-3),P53,P57,and its pro-apoptotic gene(Bax)was determined using Western blotting.Adenoviruses expressing a low level of si-miR-222-3 p and HIPK2(si-pcD-HIPK2)were co-transfected,and cells were divided into an si-miR-222-3 p+si-pcD-HIPK2 group,an si-NC+si-pcD-HIPK2 group,an si-miR-222-3 p+si-pcD-NC group,an si-NC+si-pcD-NC group,and a control group.The expression of miR-222-3 p and HIPK2 mRNA and the rate of apoptosis were determined.Results The expression of miR-222-3 p(1.62±0.15 and 2.26±0.25)and HIPK2(2.42±0.26 and 1.66±0.24)in HPV-positive and HPV-negative cervical cancer tissues increased compared to that in normal cervical tissues(1.08±0.09,1.01±0.08)(P<0.05).The expression of miR-222-3 p in HPV-positive cervical cancer increased(1.62±0.15 vs 2.26±0.25)compared to that in HPV-negative cervical cancer tissues(P<0.05),and the expression of HIPK2 decreased(2.42±0.26 vs 1.66±0.24)(P<0.05).The rate of apoptosis(31.29±3.35 and 32.33±3.24)and the expression of HIPK(2.62±0.25 and 2.76±0.27),capase-3(1.83±0.18 and 1.86±0.18),P53(1.75±0.17 and 1.79±0.16),P57(1.80±0.15 and 1.94±0.19),and Bax(1.96±0.19 and 1.94±0.18)protein increased in the si-miR-222-3 p group and the Ad-HIPK2 group compared to the rate of apoptosis and levels of expression in the control group(10.01±0.60,1.61±0.16,1.09±0.12,1.07±0.09,1.06±0.10,1.05±0.09,100.00±0.00,and 2.28±0.21)(P<0.05),while the rate of proliferation decreased(71.42±7.15 and 72.46±7.26)(P<0.05).The expression of miR-222-3 p(1.42±0.15)decreased in the si-miR-222-3 p group(P<0.05),but there was no difference in its expression in the Ad-HIPK2 group(2.46±0.26)(P<0.05).There were no significant differences in the above indexes in the si-NC group,the Ad-NC group,and the control group(P>0.05).The rate of apoptosis(2.21±0.40)in the si-NC+si-pcD-HIPK2 group decreased compared to that in the control group(10.09±1.10)(P<0.05).The rate of apoptosis(32.13±3.28)in the si-miR-222-3 p+si-pcD-NC group increased(P<0.05).The rate of apoptosis(10.19±1.29)in the si-miR-222-3 p+si-pcD-HIPK2 group was lower than that in the si-miR-222-3 p+si-pcD-NC group(32.13±3.28)(P<0.05).There were no significant differences in that index in the si-NC+si-pcD-NC group(10.03±1.16)and the control group(10.09±1.10)(P>0.05).Conclusion:The expression of miR-222-3 p was abnormally higher in HPV-positive cervical cancer tissues.Inhibiting the expression of miR-222-3 p can promote the apoptosis of cervical cancer cells infected with HPV by targeting up-regulation of the expression of HIPK2.