印染外排废水中溶解性有机质的荧光特性

Fluorescence characteristics of dissolved organic matter in textile-dyeing effluents

通过三维荧光(EEM)光谱技术,结合平行因子分析(PARAFAC)及荧光区域积分法(FRI),对我国珠三角的五家印染废水处理厂外排废水的溶解性有机质(DOM)进行定性及定量表征。五组水样DOM的EEM光谱中类蛋白荧光峰最为明显,结合荧光指数(FI),自生源指标(BIX)及腐殖化指标(HIX),发现DOM的腐殖质主要来源于微生物的活动。基于PARAFAC的结果发现印染外排废水DOM的构成组分可分为类蛋白荧光组分C1(Em/Ex=320/275 nm)和C2(Em/Ex=350/225,275 nm),及类腐殖质荧光组分C3(Em/Ex=460/250,300 nm)。不同组分之间的显著相关性表明他们具有同源或同结构相似性,其中C2与C3之间相关性最高(r=0.7949),其次是C1与C2(r=0.7936),而C1与C3之间相关性不显著(P>0.05)。结合FRI,发现外排废水中DOM的有机物构成以溶解性微生物代谢产物和芳香性蛋白类为主,类腐殖质的含量相对较低。三维荧光光谱结合平行因子分析和荧光区域积分法,能有效监测及分析外排废水中低浓度有机物的具体信息,可以作为一种有效的水质监测手段。

Based on parallel factor analysis(PARAFAC)and fluorescence regional integration(FRI),excitation emission matrix(EEM)spectroscopy was used to qualitatively and quantitatively characterize dissolved organic matter(DOM)in textile-dyeing effluents.The protein-like fluorescence peaks in the EEM spectroscopy of DOM were more obvious.Combining the fluorescence index(FI),biological index(BIX)and the humification index(HIX),it was found that the source of DOM was mainly microbial activity.Based on the results of PARAFAC,it was found that DOM can be divided into protein-like components C1(Em/Ex=320/275 nm)and C2(Em/Ex=350/225,275 nm),and humic-like fluorescent component C3(Em/Ex=460/250,300 nm).Significant correlations between different components indicated that they were homologous or homostructured.The correlation between C2 and C3 was the highest(r=0.7949),followed by C1 and C2(r=0.7936),while the correlation between C1 and C3 was not significant(P>0.05).Combined with FRI,it was found that the organic matter composition of DOM was mainly composed of soluble microbial metabolites and aromatic proteins,the content of humic substances was relatively low.The EEM spectrum combined with PARAFAC and FRI could effectively monitor and analyze the specific information of low-concentration organic matter in the effluents.It could be used as an effective technical means for water quality monitoring of the treatment plant.