摘要
Dear Editors,CRISPR/Cas9-mediated genome editing techniques have triggered a revolution in biology research(Jinek et al.,2012).Cas9 coupled with guide RNAs cuts DNA at precise positions,and the resulting double stranded breaks(DSB)were effectively repaired by non-homologous end joining(NHEJ)pathway in higher eukaryotic cells,including animals and plants.
基金
supported by the National Transgenic Science and Technology Program(2019ZX08010-003)
National Natural Science Foundation of China(31872933)。
