老年冠心病患者血清NOX2、NOX4 mRNA水平变化及其与预后的关系

Changes of serum NOX2 and NOX4 mRNA levels in elderly patients with coronary heart disease and their relationship with prognosis

目的探究还原型烟酰胺腺嘌呤二核苷酸磷酸氧化酶(NOX)2、NOX4 mRNA在老年冠心病(CHD)患者血清中的水平变化及其与预后的关系。方法选取该院收治的老年CHD患者146例为CHD组,另选取体检健康老年人146例为对照组。采用实时荧光定量PCR检测血清NOX2、NOX4 mRNA水平。比较CHD组与对照组、不同预后老年CHD患者的血清NOX2、NOX4 mRNA及丙二醛(MDA)、超氧化物歧化酶(SOD)水平;分析老年CHD患者血清NOX2、NOX4 mRNA水平与MDA、SOD水平的相关性;分析老年CHD患者预后的影响因素;分析血清NOX2、NOX4 mRNA水平对老年CHD患者预后的预测价值。结果CHD组血清NOX2、NOX4 mRNA及MDA水平均明显高于对照组(P<0.05),血清SOD水平明显低于对照组(P<0.05)。预后不良患者血清NOX2、NOX4 mRNA及MDA水平均明显高于预后良好患者(P<0.05),血清SOD水平明显低于预后良好患者(P<0.05)。老年CHD患者血清NOX2、NOX4 mRNA水平与MDA水平呈正相关(P<0.05),与SOD水平呈负相关(P<0.05)。血清NOX2、NOX4 mRNA及MDA水平升高为老年CHD患者预后的独立危险因素(P<0.05),血清SOD水平升高为老年CHD患者预后的独立保护因素(P<0.05)。血清NOX2、NOX4 mRNA联合预测老年CHD患者不良预后的曲线下面积为0.943。结论老年CHD患者血清NOX2、NOX4 mRNA均呈高表达,NOX2、NOX4 mRNA水平升高为老年CHD患者预后的影响因素,且有望成为评估老年CHD患者预后的预测指标。

Objective To explore the changes in serum levels of reduced nicotinamide adenine dinucleotide phosphate oxidase(NOX)2 and NOX4 mRNA in elderly patients with coronary heart disease(CHD)and their relationship with prognosis.Methods A total of 146 elderly CHD patients admitted to the hospital were selected as the CHD group,and 146 healthy elderly patients were selected as the control group.Real-time fluorescent quantitative PCR was used to detect serum NOX2 and NOX4 mRNA levels.Compared the serum levels of NOX2 mRNA,NOX4 mRNA,malondialdehyde(MDA)and superoxide dismutase(SOD)between CHD group and control group,elderly CHD patients with different prognosis.Analyzed the correlation between serum NOX2,NOX4 mRNA levels and MDA,SOD levels in elderly CHD patients.Analyzed the factors affecting the prognosis of elderly CHD patients.Analyzed the predictive value of serum NOX2 and NOX4 mRNA levels on the prognosis of elderly CHD patients.Results The levels of serum NOX2,NOX4 mRNA and MDA in the CHD group were significantly higher than those in the control group(P<0.05),and the serum SOD level was significantly lower than that in the control group(P<0.05).The levels of serum NOX2,NOX4 mRNA and MDA in patients with poor prognosis were significantly higher than those in patients with good prognosis(P<0.05),and serum SOD level was significantly lower than that in patients with good prognosis(P<0.05).The serum levels of NOX2 and NOX4 mRNA in elderly CHD patients were positively correlated with MDA level(P<0.05),and negatively correlated with SOD level(P<0.05).The increase of serum NOX2,NOX4 mRNA and MDA levels were independent risk factors for prognosis of elderly CHD patients(P<0.05),and the increase of serum SOD level was the independent protective factor for prognosis of elderly CHD patients(P<0.05).The area under the curve for the combined prediction of serum NOX2 and NOX4 mRNA for the poor prognosis of elderly CHD patients was 0.943.Conclusion The serum levels of NOX2 and NOX4 mRNA in elderly CHD patients are highly expressed.The levels of NOX2 and NOX4 mRNA may be influencing factors for the prognosis of elderly CHD patients,and they are expected to become prediction indicators for evaluating the prognosis of elderly CHD patients.