针刺对腰椎间盘突出症大鼠脊髓和下丘脑中P物质及其受体表达的影响

Effects of acupuncture on the expression of substance P and its receptor in lumbar disc herniation rats

目的:探索针刺对腰椎间盘突出症大鼠脊髓、下丘脑中P物质及其受体表达的影响。方法:SPF级雄性SD大鼠40只,按照随机数字表分为正常组、模型组、肾俞组、非经非穴组,每组10只。正常组正常饲养,不予针刺,其余组采用非压迫性(自体髓核)腰椎间盘髓核突出模型的造模方法。造模3d后,肾俞组针刺大鼠肾俞穴,非经非穴组针刺大鼠非经非穴,两组均每天1次,连续治疗5次。观察各组造模前后及治疗后热痛刺激缩爪阈值;ELISA法测定脊髓和下丘脑组织中P物质含量;免疫组化法检测脊髓组织中P物质受体NK-1R、NK-2R、NK-3R的表达;RT-PCR方法检测脊髓组织中P物质受体NK-IR、NK-2R、NK-3R mRNA的表达。结果:针刺后各组热痛缩爪阈值上升;与模型组比较,肾俞组脊髓和下丘脑P物质含量显著下降(P<0.05),其受体NK-1R、NK-2R、NK-3R mRNA表达水平显著降低(P<0.05);与肾俞组比较,非经非穴组脊髓和下丘脑P物质含量显著升高(P<0.05)。结论:针刺肾俞穴能够缓解大鼠对痛觉的敏感,减轻大鼠疼痛,各组治疗后大鼠脊髓和下丘脑P物质含量显著降低,通过针刺疗效分析,说明针刺经穴对治疗腰椎间盘突出症有较好的治疗作用。

Objective:To explore the effects of acupuncture on the expression of substance P and its receptor in lumbar disc herniation rats.Methods:A total of 40 SPF grade male SD rats were divided into normal group,model group,Shenshu(BL 23)group,non-meridian and non-acupoint group according to the random number table,10 rats in each group.In the normal group,normal feeding was given and no acupuncture was given,the other groups were treated with noncompression(autologous nucleus pulposus)lumbar disc herniation model.Three days after the establishment of the model,the rats of Shenshu(BL 23)group were acupunctured at Shenshu(BL 23),the non-meridian and non-acupoint group was acupunctured at non-meridian and non-acupoint,once a day and treated five times.The content of SP in spinal cord and hypothalamus were determined by ELISA,and the expression of SP receptor NK-1 R,NK-2 R and NK-3 R mRNA were detected by reverse transcription gradient PCR.Results:After acupuncture,the paw retraction threshold of each group increased;Compared with the model group,the content of substance P in spinal cord and hypothalamus of Shenshu(BL 23)group decreased significantly(P<0.05),the mRNA expression levels of NK-1 R,NK-2 R and NK-3 R were significantly decreased(P<0.05);Compared with Shenshu(BL 23)group,the content of substance P in spinal cord and hypothalamus of non-meridian and non-acupoint group was significantly increased(P<0.05).Conclusion:Acupuncture at Shenshu(BL 23)can alleviate the sensitivity to pain and the pain of rats,the contents of SP in spinal cord and hypothalamus of every treatment group decreased significantly.The analysis shows that acupuncture has better therapeutic effect on lumbar disc herniation.