HIV/AIDS肺脾气虚证患者外周血中调节性T细胞和髓源性抑制细胞的变化

Changes of Treg and MDSCs in peripheral blood of HIV/AIDS patients with syndrome of qi deficiency of lung and spleen

目的:探讨HIV/AIDS肺脾气虚证患者外周血中的调节性T细胞(Treg)、髓源性抑制细胞(MDSCs)的变化。方法:收集27例HIV/AIDS肺脾气虚证患者、5例HIV无证可辨者及10名健康人抗凝全血,流式细胞术检测各样本T淋巴细胞亚群、Treg和MDSCs数量,分析各组T淋巴细胞亚群、Treg和MDSCs的变化。结果:肺脾气虚证患者CD_(4)^(+)T淋巴细胞数量较无证可辨者和健康人显著下降(P<0.05);HIV感染人群Treg和MDSCs数量较健康人群显著升高(P<0.05),尤其以肺脾气虚证组免疫抑制性细胞比例最高;肺脾气虚证患者体内Treg和M-MDSCs水平与CD_(4)^(+)T淋巴细胞绝对计数呈负相关。结论:HIV/AIDS肺脾气虚证患者体内免疫抑制性细胞Treg和MDSCs水平上升,且其变化与HIV疾病进展相关,可作为中医证候客观化和标准化的辅助指标。

Objective:To explore the changes of regulatory T cells(Treg)and myeloid suppressor cells(MDSCs)in peripheral blood of HIV/AIDS patients with lung and spleen qi deficiency syndrome.Methods:Whole blood samples were collected from 27 HIV/AIDS patients with lung-spleen deficiency syndrome,5 HIV patients without obvious clinical symptoms&TCM syndromes and 10 healthy people.The number of T lymphocyte subsets,Treg and MDSCs were detected by flow cytometry,and the changes of T lymphocyte subsets,Treg and MDSCs in different groups were analyzed.Results:The number of CD_(4)^(+)T lymphocytes in the group of deficiency of lung and spleen qi was significantly lower than that in the group without obvious clinical symptoms&TCM syndromes and the healthy control group(P<0.05);The number of Treg and MDSCs in HIV-infected people were significantly higher than that in healthy people(P<0.05),especially in the group of deficiency of lung and spleen qi;The levels of Treg and M-MDSCs were negatively correlated with the absolute counts of CD_(4)^(+)T lymphocytes.Conclusion:The levels of Treg and MDSCs in HIV/AIDS patients with deficiency of lung and spleen qi increase,and their changes are related to the progress of HIV disease,which could be used as an auxiliary index for the quantification and standardization of TCM syndromes.