酿酒葡萄品种对绿盲蝽抗性的筛选及其挥发物初步鉴定

Screening of Resistance of Wine Grape Varieties to Apolygus Lucorum and Preliminary Identification of its Volatiles

为了确定绿盲蝽在葡萄园区的取食和产卵行为,以及不同酿酒葡萄品种释放的挥发性物质成分,在田间对不同品种酿酒葡萄叶片的受害情况和各品种酿酒葡萄上的绿盲蝽越冬卵量进行调查。结果表明,不同葡萄品种对绿盲蝽的抗性不同,其中玛瑟兰对绿盲蝽抗性最强,抗性指数为5.0,小味儿多对绿盲蝽抗性最弱,抗性指数为1.0;绿盲蝽在不同葡萄品种上的越冬卵量存在差异,分析表明不同品种酿酒葡萄叶片受害指数与绿盲蝽越冬卵量之间无相关性。采用顶空采样技术,分析不同抗性酿酒葡萄苗释放的挥发性物质含量与种类的差异,5个不同品种的酿酒葡萄共鉴定出53种挥发性成分,不同抗性酿酒葡萄释放的挥发物不尽相同,其中苯酚、顺-2-戊烯-1-醇、长叶烯等化合物为高抗植物玛瑟兰所特有,邻苯二甲酸二甲酯、正十三烷、乙二膦酸等化合物为高感植物特有。

The damage of leaves of different wine grape varieties and the amount of overwintering eggs of Apolygus lucorum on wine grape varieties were investigated to know the feeding and oviposition behavior of Apolygus lucorum in grape gardens and the volatile compounds released from different wine grape varieties.The results showed that the resistance of different grape varieties to Apolygus lucorum was different,among which Marselan had the strongest resistance,with resistance index of 5.0,while Petit Verdot had the weakest resistance to Apolygus lucorum,with the resistance index of 1.0.The number of Apolygus lucorum overwintering eggs on different grape varieties were different.The results showed that there was no correlation between the damage index of different wine grape leaves and the number of Apolygus lucorum overwintering eggs.Headspace sampling technique was used to analyze the content and species of volatile compounds released by grape seedlings with different resistance.A total of 53 volatile components were identified from 5 different varieties of wine grape.The volatiles released by different resistant grape seedlings were different.The highly resistant plant mathelan was featured with compounds such as phenol,cis-2-Penten-1-ol and longifolene,while the highly sensitive plants were featured with dimethyl phthalate,n-tridecane,and etidronic acid.