摘要
从酸模Rumex acetosa内生真菌Fusarium sp.HU0298玉米发酵物的活性流分中分离鉴定了一个主要成分:亚油酸;结合活性流分的气相色谱(GC)组分分析和活性评价发现亚油酸是该发酵物的主要抗耐甲氧西林金黄色葡萄球菌(MRSA)成分;通过对亚油酸和多个其他脂肪酸标准品的活性测试探究了脂肪酸化学结构和与抗MRSA活性之间的构效关系,发现其抗菌活性不仅与脂肪性的链长有关,也与其不饱和度密切相关,碳链上顺式双键是影响抗菌活性关键基团。研究结果初步阐明了Fusarium sp.HU0298玉米发酵物的抗MRSA活性物质基础,丰富了镰刀菌抗细菌活性代谢产物多样性,为研发安全有效的抗MRSA药物提供了新的思路。
Linoleic acid was isolated as the major compound from the active fractions of corn fermented with an Rumex acetosa endophyte Fusarium sp.HU0298.GC analysis and activity evaluation proved that linoleic acid was the main active component.Structure-activity relationship between fatty acids’structures and anti-MRSA activities showed that the antibacterial activity against MRSA of different fatty acids was closely related to their chain length and unsaturated level.The results revealed the active substance of anti-MRSA of Fusarium sp.HU0298,and enriched the diversity of the antibacterial metabolites of Fusarium fungi,providing a new idea for developing the safe and effective drugs against MRSA.
作者
李丰名
陈嘉颖
吕镇城
徐良雄
黄日明
LI Feng-Ming;CHEN Jia-Ying;LV Zhen-Cheng;XU Liang-Xiong;HUANG Ri-Ming(College of Food Sciences,South China Agricultural University,Guangzhou,Guangdong 510631,China;School of Life Sciences,Huizhou University,Huizhou,Guangdong 516007,China)
出处
《菌物学报》
CAS
CSCD
北大核心
2021年第5期1140-1147,共8页
Mycosystema
基金
国家自然科学基金(31772032)
惠州学院自然科学研究项目(20170406122437260)。