枯草芽孢杆菌发酵制备花生饼粕、花生茎叶混合饲料条件优化研究

Optimization of Peanut Meal and Peanut Stem and Leaf Mixed Feed by Fermentation of Bacillus subtilis

以花生饼粕、花生茎叶混合饲料为原料,筛选最佳枯草芽孢杆菌培养条件为目标,研究料液比、接种量、发酵温度、发酵时间等因素对枯草芽孢杆菌培养的影响。结果表明,最优培养条件:加水量(料液比)1∶0.83、培养时间62 h、接菌量0.78 g、培养温度30℃,在该条件下所得枯草芽孢杆菌芽孢数为(195.222±0.003)。

Taking peanut meal and peanut stem and leaf mixed feed as raw materials,screening the best Ba-cillus subtilis culture conditions as the goal,to study the influence of factors such as the ratio of material to liq-uid,inoculum,fermentation temperature,and fermentation time on the culture of Bacillus subtilis.The results showed that the optimal culture conditions:water addition(material-to-liquid ratio)1∶0.83,culture time 62 h,inoculation amount 0.78 g,culture temperature 30℃,the number of Bacillus subtilis spores obtained un-der these conditions is(195.222±0.003).